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Gene Expression Data
Assay Details
Assay
Reference: J:326556 Wang Y, et al., DDX1 vesicles control calcium-dependent mitochondrial activity in mouse embryos. Nat Commun. 2022 Jul 1;13(1):3794
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7530655
Gene symbol: Ddx1
Gene name: DEAD box helicase 1
Antibody: anti-DDX1 (2910)
Detection system: other - see notes
Assay notes: Primary antibody conjugated with Atto 550.
Results
Specimen 1a: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 1a Magnified , 1a DDX1 Expression aggregates consisting of a ring-like cluster.
Specimen S1a 1-cell: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted S1a 1-cell Expression in aggregates.
Specimen S1a 2-cell: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S1a 2-cell Expression in aggregates forming ring-like structures at 2-cell stage.
Specimen S1a 4-cell: embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted S1a 4-cell Aggregates become larger, denser and less ring-like with embryonic development.
Specimen S1a Morula: embryonic day 3.0 (more )
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted S1a Morula Aggregates become larger, denser and less ring-like with embryonic development.
Specimen S1a Blastocysts: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted S1a Blastocysts Aggregates are mostly observed around nucleus at blastocyst stage.
Specimen S1b Mid Section: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S1b Mid Section Expression primarily found at subplasmalemma cytoplasm.
Specimen S1b Top Section: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S1b Top Section Expression primarily found at subplasmalemma cytoplasm.
Specimen S3a DDX1: embryonic day 1.0 (more )
Note: Double labeled: magenta - Ddx1; green - Rps6 (assay MGI:7530657).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S3a Merge , S3a DDX1 No co-expression with Rps6.
Specimen S3b DDX1 (top): embryonic day 1.0 (more )
Note: Double labeled: magenta - Ddx1; green - Mrpl42 (assay MGI:7530658).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S3b Merge (top) , S3b DDX1 (top) No co-expression with Mrpl42.
Specimen S3b DDX1 (middle): embryonic day 1.0 (more )
Note: Double labeled: magenta - Ddx1; green - Mrpl44 (assay MGI:7530659).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S3b Merge (middle) , S3b DDX1 (middle) No co-expression with Mrpl44.
Specimen S3b DDX1 (bottom): embryonic day 1.0 (more )
Note: Double labeled: magenta - Ddx1; green - Mrps27 (assay MGI:7530661).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S3b Merge (bottom) , S3b DDX1 (bottom) No co-expression with Mrps27.
Specimen S7a DDX1: embryonic day 1.0 (more )
Note: Double labeled: magenta - Ddx1; green - Cpeb1 (assay MGI:7530662).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S7a DDX1 , S7a Merge Co-expression with Cpeb1.
Specimen S7b DDX1: embryonic day 1.0 (more )
Note: Double labeled: magenta - Ddx1; green - Cpsf2 (assay MGI:7530663).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S7b Merge , S7b DDX1 Co-expression with Cpsf2.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory