Notch1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:339600 Hermosilla Aguayo V, et al., ESCRT-dependent control of craniofacial morphogenesis with concomitant perturbation of NOTCH signaling. Dev Biol. 2023 Aug 10;503:25-42
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7529609
Gene symbol:	Notch1
Gene name:	notch 1

Antibody:	C458.2H
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to DyLight 488

Results

10B +/+ top ©

Specimen 10B Vps25^+/+: embryonic day 10.5 (more

)
Note: Double labeled: green - Notch1; magenta - Lamp1 (assay MGI:7529608).

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process	Present	Regionally restricted	10B +/+ top , 10B +/+ bottom	Expression of Notch1 extracellular domain co-localized with Lamp1 in late endosomes.

Specimen 10B Vps25^ENU/ENU: embryonic day 10.5; Vps25^m1Lis/Vps25^m1Lis (more

)
Note: Double labeled: green - Notch1; magenta - Lamp1 (assay MGI:7529608).

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process	Present	Regionally restricted	10B ENU/ENU bottom , 10B ENU/ENU top	Expression of Notch1 extracellular domain co-localized with Lamp1 in late endosomes. Notch1 extracellular domain was similarly present in the mutant compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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