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Gene Expression Data
Assay Details
Assay
Reference: J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520523
Gene symbol: Pard3
Gene name: par-3 family cell polarity regulator
Antibody: anti-Par3 (07-330)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to DyLight
Results
Specimen S3E Par3 Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: blue - Pard3; red - Pcnt (assay MGI:7520526).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S3E Control Merge , S3E Control Par3
Specimen S3E Par3 DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: blue - Pard3; red - Pcnt (assay MGI:7520526).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S3E DKO Merge , S3E DKO Par3 Localization of PAR3 polarity complex similar to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory