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Gene Expression Data
Assay Details
Assay
Reference: J:310864 Zainolabidin N, et al., Distinct Activities of Tfap2A and Tfap2B in the Specification of GABAergic Interneurons in the Developing Cerebellum. Front Mol Neurosci. 2017;10:281
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520303
Gene symbol: Tfap2a
Gene name: transcription factor AP-2, alpha
Antibody: clone 3B5
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 1 Present Regionally restricted 1A Not expressed in the rhombic lip and the intermediate domain, where the first cerebellar neurons were generated.

Specimen 1C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebellum white matter Present Regionally restricted 1C Expressed in GABAergic precursors in the underlying white matter where inhibitory neurons transit after exiting the ventricular zone.

Specimen 1E: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebellum white matter Present Regionally restricted 1E Expressed in GABAergic precursors in the underlying white matter where inhibitory neurons transit after exiting the ventricular zone.

Specimen 1G: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Present Regionally restricted 1G Expressed in a subset of cells.
TS27: cerebellum white matter Present 1G

Specimen 1I: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: cerebellum internal granule cell layer Present Regionally restricted 1I Expressed in the lower half.
TS28: cerebellum molecular layer Present Regionally restricted 1I Expressed in the lower half.
TS28: cerebellum Purkinje cell layer Present 1I

Specimen 1K: postnatal day 150 (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 1K
TS28: cerebellum internal granule cell layer Present 1K
TS28: cerebellum molecular layer Present 1K

Specimen 2A/C: postnatal day 60 (more )
Note: Double labeled: green - Tfap2a; red - Pvalb (assay MGI:7520307).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 2C , 2A Coexpressed with Pvalb.
TS28: cerebellum molecular layer cerebellar stellate cell Present 2A , 2C Coexpressed with Pvalb.
TS28: cerebellum molecular layer cerebellum basket cell Present 2A , 2C Coexpressed with Pvalb.
TS28: cerebellum internal granule cell layer Present Single cells 2C , 2A

Specimen 2D/F: postnatal day 60 (more )
Note: Double labeled: green - Tfap2a; red - Grm2 (assay MGI:7520308).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum internal granule cell layer cerebellar Golgi cell Present Single cells 2D , 2F Coexpressed with Grm2.
TS28: cerebellum Purkinje cell layer Present 2F , 2D
TS28: cerebellum molecular layer Present 2F , 2D

Specimen 2G/I: postnatal day 60 (more )
Note: Double labeled: green - Tfap2a; red - Rbfox3 (assay MGI:7520309).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum internal granule cell layer cerebellar granule cell Absent 2I , 2G Not coexpressed with Rbfox3.
TS28: cerebellum internal granule cell layer cerebellar Golgi cell Present Single cells 2G , 2I
TS28: cerebellum Purkinje cell layer Present 2G , 2I
TS28: cerebellum molecular layer Present 2G , 2I

Specimen S2A: postnatal adult (more )
Note: Triple labeled: green - Tfap2a; blue - Gad1 (assay MGI:7520319); red - Map2 (assay MGI:7520320).
Structure Cell Type Level Pattern Image Note
TS28: fastigial nucleus cerebellar inhibitory GABAergic interneuron Present Regionally restricted S2A Tfap2A , S2A Coexpressed with Map2 and Gad1 in small GABAergic neurons in medial cerebellar nucleus.

Specimen S2C: postnatal adult (more )
Note: Triple labeled: green - Tfap2a; blue - Gad1 (assay MGI:7520319); red - Map2 (assay MGI:7520320).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum interpositus nucleus cerebellar inhibitory GABAergic interneuron Present Regionally restricted S2C Tfap2A , S2C Coexpressed with Map2 and Gad1 in small GABAergic neurons.

Specimen S2E: postnatal adult (more )
Note: Triple labeled: green - Tfap2a; blue - Gad1 (assay MGI:7520319); red - Map2 (assay MGI:7520320).
Structure Cell Type Level Pattern Image Note
TS28: dentate nucleus cerebellar inhibitory GABAergic interneuron Present Regionally restricted S2E Tfap2A , S2E Coexpressed with Map2 and Gad1 in small GABAergic neurons in lateral deep cerebellar nucleus.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory