About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:276652 Fang Q, et al., Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function. Sci Rep. 2019 Mar 4;9(1):3357
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506983
Gene symbol: Dlg4
Gene name: discs large MAGUK scaffold protein 4
Antibody: anti-PSD-95
Assay notes: Fixation with 4% polyoxymethylene.
Results
Specimen 5a PSD95 P14 WT: postnatal day 14 (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present Patchy 5a P14 WT PSD95 , 5a P14 WT Merged inset , 5a P14 WT Merged , 5a P14 WT PSD95 inset Expression in post-synaptic ribbon terminals. Expression concentrated at terminus and plasma membrane of IHCs.
Specimen 5a PSD95 P14 DKO: postnatal day 14; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present Punctate 5a P14 DKO Merged inset , 5a P14 DKO PSD95 , 5a P14 DKO Merged , 5a P14 DKO PSD95 inset No difference in expression compared to wild type from apical to basal turns.
Specimen 5a PSD95 P30 WT: postnatal day 30 (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present Punctate 5a P30 WT Merged , 5a P30 WT PSD95 inset , 5a P30 WT PSD95 , 5a P30 WT Merged inset Expression in post-synaptic ribbon terminals. Expression concentrated at terminus and plasma membrane of IHCs.
Specimen 5a PSD95 P30 DKO: postnatal day 30; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present Punctate 5a P30 DKO Merged , 5a P30 DKO PSD95 , 5a P30 DKO Merged inset , 5a P30 DKO PSD95 inset No difference in expression compared to wild type from apical to basal turns.
Specimen S4 PSD95 APEX P14 WT: postnatal day 14 (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_APEX P14 WT PSD95 , S4_APEX P14 WT PSD95 inset , S4_APEX P14 WT Merged Inset , S4_APEX P14 WT Merged
Specimen S4 PSD95 APEX P14 DKO: postnatal day 14; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_APEX P14 DKO Merged , S4_APEX P14 DKO Merged inset , S4_APEX P14 DKO PSD95 , S4_APEX P14 DKO PSD95 inset
Specimen S4 PSD95 APEX P30 WT: postnatal day 30 (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_APEX P30 WT PSD95 , S4_APEX P30 WT Merged , S4_APEX P30 WT Merged inset , S4_APEX P30 WT PSD95 inset
Specimen S4 PSD95 APEX P30 DKO: postnatal day 30; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_APEX P30 DKO Merged inset , S4_APEX P30 DKO Merged , S4_APEX P30 DKO PSD95 , S4_APEX P30 DKO PSD95 inset
Specimen S4 PSD95 MID P14 WT: postnatal day 14 (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_MID P14 WT PSD95 inset , S4_MID P14 WT PSD95 , S4_MID P14 WT Merged , S4_MID P14 WT Merged inset
Specimen S4 PSD95 MID P14 DKO: postnatal day 14; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_MID P14 DKO Merged , S4_MID P14 DKO PSD95 , S4_MID P14 DKO PSD95 inset , S4_MID P14 DKO Merged inset
Specimen S4 PSD95 MID P30 WT: postnatal day 30 (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_MID P30 WT PSD95 inset , S4_MID P30 WT Merged inset , S4_MID P30 WT Merged , S4_MID P30 WT PSD95
Specimen S4 PSD95 MID P30 DKO: postnatal day 30; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: green - Dlg4 (PSD95); red - Ctbp2 (assay MGI:7506982).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Present S4_MID P30 DKO PSD95 , S4_MID P30 DKO Merged , S4_MID P30 DKO PSR95 inset , S4_MID P30 DKO Merged inset
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory