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Gene Expression Data
Assay Details
Assay
Reference: J:276652 Fang Q, et al., Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function. Sci Rep. 2019 Mar 4;9(1):3357
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506975
Gene symbol: Limk1
Gene name: LIM domain kinase 1
Antibody: anti-LIMK1 (sc-8387)
Assay notes: Fixation with 4% polyoxymethylene.
Results
Specimen 1a Limk1 P21: postnatal day 21 (more )
Note: Double labeled: red - Limk1; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS28: cochlea epithelium cochlea auditory hair cell Present 1a P21 z-stack (left) , 1a LIMK1 P21 , 1a P21 Merged (left) Expression in cytoplasm of hair cells and supporting cells. No difference in expression from apical to basal turns.
TS28: cochlea epithelium supporting cell of cochlea Present 1a P21 z-stack (left) , 1a LIMK1 P21 , 1a P21 Merged (left) Expression in cytoplasm of hair cells and supporting cells. No difference in expression from apical to basal turns.
Specimen 1a Limk1 P30: postnatal day 30 (more )
Note: Double labeled: red - Limk1; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlea auditory hair cell Present 1a P30 Merged (left) , 1a P30 z-stack (left) , 1a LIMK1 P30 Expression in cytoplasm of hair cells and supporting cells. No difference in expression from apical to basal turns.
TS28: cochlea supporting cell of cochlea Present 1a P30 Merged (left) , 1a P30 z-stack (left) , 1a LIMK1 P30 Expression in cytoplasm of hair cells and supporting cells. No difference in expression from apical to basal turns.
Specimen S2 LImk1 P21: postnatal day 21 (more )
Note: Double labeled: red - Limk1; blue - Sox2 (assay MGI:7506978).
Structure Cell Type Level Pattern Image Note
TS28: cochlea Deiter's cell Present S2_LIMK1 P21 , S2_P21 z-stack (left) , S2_P21 Merged (left)
TS28: cochlea pillar cell Present S2_LIMK1 P21 , S2_P21 z-stack (left) , S2_P21 Merged (left)
Specimen S2 LImk1 P30: postnatal day 30 (more )
Note: Double labeled: red - Limk1; blue - Sox2 (assay MGI:7506978).
Structure Cell Type Level Pattern Image Note
TS28: cochlea Deiter's cell Present S2_P30 Merged (left) , S2_P30 z-stack (left) , S2_LIMK1 P30
TS28: cochlea pillar cell Present S2_P30 Merged (left) , S2_P30 z-stack (left) , S2_LIMK1 P30
Specimen 2a Limk1 Cytoplasm WT: postnatal day 3 (more )
Note: Double labeled: red - Limk1; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS27: cochlea epithelium cochlea auditory hair cell Present 2a Cytoplasm WT Merged (left) , 2a Cytoplasm WT LIMK1
Specimen 2a Limk1 Cytoplasm DKO: postnatal day 3; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: red - Limk1; green - Myo7a (assay MGI:7506974).
Structure Level Pattern Image Note
TS27: cochlea epithelium Absent 2a Cytoplasm DKO LIMK1 , 2a Cytoplasm DKO Merged (left)
Specimen 2a Limk1 Nucleus WT: postnatal day 3 (more )
Note: Double labeled: red - Limk1; green - Myo7a (assay MGI:7506974).
Structure Cell Type Level Pattern Image Note
TS27: cochlea epithelium cochlea auditory hair cell Present 2a Nucleus WT Merged (left) , 2a Nucleus WT LIMK1
Specimen 2a Limk1 Nucleus DKO: postnatal day 3; Limk1tm1Zpj/Limk1tm1Zpj, Limk2tm1.1Tnak/Limk2tm1.1Tnak (more )
Note: Double labeled: red - Limk1; green - Myo7a (assay MGI:7506974).
Structure Level Pattern Image Note
TS27: cochlea epithelium Absent 2a Nucleus DKO LIMK1 , 2a Nucleus DKO Merged (left)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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