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Gene Expression Data
Assay Details
Assay
Reference: J:336449 Haerlingen B, et al., Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification. Development. 2023 May 15;150(10)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7505625
Gene symbol: Pax8
Gene name: paired box 8
Antibody: anti-Pax8 (PA0300)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 7A: embryonic day 7.5 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS11: non-neural ectoderm Strong 7A
TS11: embryo endoderm Strong Regionally restricted 7A Expression is in small clusters of epithelial cells of the foregut endoderm.
Specimen 7B: embryonic day 7.75 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS12: non-neural ectoderm Strong 7B
TS12: foregut diverticulum endoderm Strong Regionally restricted 7B Expression is in small clusters of epithelial cells of the foregut endoderm.
Specimen 7E: embryonic day 7.5 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS11: embryo endoderm Present Regionally restricted 7E Expression is in patches in lateral regions of the foregut pocket. Absent in the dorsal foregut epithelium.
Specimen 7F: embryonic day 7.75 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS12: foregut diverticulum endoderm Present Regionally restricted 7F Expression is in patches in lateral and medial regions of the ventral foregut epithelium. Absent in the dorsal foregut epithelium.
Specimen 7G: embryonic day 7.75 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS12: foregut diverticulum endoderm Present Regionally restricted 7G Expression was limited to lateral foregut epithelium in more caudal positions. Absent in the dorsal foregut epithelium.
Specimen 7I: embryonic day 8.0 (more )
Note: This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present Regionally restricted 7I There is a large anterior-posterior expansion of the expression domain.
Specimen 7J: embryonic day 8.0 (more )
Note: This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present Regionally restricted 7J There is a large anterior-posterior expansion of the expression domain.
Specimen 7K: embryonic day 8.0 (more )
Note: This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present Regionally restricted 7K There is a large anterior-posterior expansion of the expression domain.
Specimen 7M: embryonic day 8.5 (more )
Note: This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS14: foregut epithelium Present Regionally restricted 7M Expression is in the ventral epithelium and in the lateral wall of the foregut along a large stretch of the anterior-posterior axis.
Specimen 7N: embryonic day 8.5 (more )
Note: This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS14: foregut epithelium Present Regionally restricted 7N Expression is in the ventral epithelium and in the lateral wall of the foregut along a large stretch of the anterior-posterior axis.
Specimen 7O: embryonic day 8.5 (more )
Note: This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS14: foregut epithelium Present Regionally restricted 7O Expression is in the ventral epithelium and in the lateral wall of the foregut along a large stretch of the anterior-posterior axis.
Specimen 7Q: embryonic day 9.0 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS14: thyroid primordium Present 7Q
Specimen 7S: embryonic day 9.5 (more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 7S
Specimen S6 left: embryonic day 8.0 (more )
Note: Theiler stage 13 morphology.
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present Regionally restricted S6_Pax8 left Expression is from ventral floor towards the endodermal pouch epithelium.
Specimen S6 middle: embryonic day 8.0 (more )
Note: Theiler stage 13 morphology.
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present Regionally restricted S6_Pax8 middle Expression is in the ventral floor and in the lateral wall of the anterior foregut tube.
Specimen S6 right: embryonic day 8.0 (more )
Note: Theiler stage 13 morphology.
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present S6_Pax8 right Expression is in the lateral epithelium of the foregut tube.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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