Gene Expression Data
Assay Details
Assay
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Reference:
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J:336449
Haerlingen B, et al., Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification. Development. 2023 May 15;150(10)
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7505625
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Gene symbol:
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Pax8
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Gene name:
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paired box 8
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Antibody:
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anti-Pax8 (PA0300)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
7A:
embryonic day 7.5
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7A:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Specimen
7B:
embryonic day 7.75
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Specimen
7E:
embryonic day 7.5
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7E:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
|
Note:
|
Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo endoderm |
Present |
Regionally restricted |
7E
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Expression is in patches in lateral regions of the foregut pocket. Absent in the dorsal foregut epithelium.
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Specimen
7F:
embryonic day 7.75
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7F:
(close )
Genetic Background:
|
C57BL/6J
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
|
Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: foregut diverticulum endoderm |
Present |
Regionally restricted |
7F
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Expression is in patches in lateral and medial regions of the ventral foregut epithelium. Absent in the dorsal foregut epithelium.
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Specimen
7G:
embryonic day 7.75
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7G:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: foregut diverticulum endoderm |
Present |
Regionally restricted |
7G
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Expression was limited to lateral foregut epithelium in more caudal positions. Absent in the dorsal foregut epithelium.
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Specimen
7I:
embryonic day 8.0
(more )
Note: This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7I:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Agarose
|
Note:
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This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
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Specimen
7J:
embryonic day 8.0
(more )
Note: This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7J:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 8.0
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Sex:
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Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Specimen
7K:
embryonic day 8.0
(more )
Note: This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7K:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 8.0
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Sex:
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Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
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This embryo had a TS13 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
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Specimen
7M:
embryonic day 8.5
(more )
Note: This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7M:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: foregut epithelium |
Present |
Regionally restricted |
7M
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Expression is in the ventral epithelium and in the lateral wall of the foregut along a large stretch of the anterior-posterior axis.
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Specimen
7N:
embryonic day 8.5
(more )
Note: This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7N:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: foregut epithelium |
Present |
Regionally restricted |
7N
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Expression is in the ventral epithelium and in the lateral wall of the foregut along a large stretch of the anterior-posterior axis.
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Specimen
7O:
embryonic day 8.5
(more )
Note: This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7O:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 8.5
|
Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
This embryo had a TS14 morphology. Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: foregut epithelium |
Present |
Regionally restricted |
7O
|
Expression is in the ventral epithelium and in the lateral wall of the foregut along a large stretch of the anterior-posterior axis.
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Specimen
7Q:
embryonic day 9.0
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7Q:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Agarose
|
Note:
|
Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
|
Specimen
7S:
embryonic day 9.5
(more )
Note: Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
Specimen
7S:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: green - Pax8; red - Cdh1 (assay MGI:7505627).
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Specimen
S6 left:
embryonic day 8.0
(more )
Note: Theiler stage 13 morphology.
Specimen
S6 left:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Theiler stage 13 morphology.
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Specimen
S6 middle:
embryonic day 8.0
(more )
Note: Theiler stage 13 morphology.
Specimen
S6 middle:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Theiler stage 13 morphology.
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Specimen
S6 right:
embryonic day 8.0
(more )
Note: Theiler stage 13 morphology.
Specimen
S6 right:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Agarose
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Note:
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Theiler stage 13 morphology.
|
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