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Gene Expression Data
Assay Details
Assay
Reference: J:334018 Janeckova E, et al., Canonical Wnt signaling regulates soft palate development by mediating ciliary homeostasis. Development. 2023 Mar 1;150(5):dev201189
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7488407
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Antibody: anti-MyoD [EPR6653-131] (ab133627)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A,E: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - Osr2 Cre reporter.
Structure Level Pattern Image Note
TS21: palatal shelf Present 1E , 1A
Specimen 2M,N: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl.
Structure Level Pattern Image Note
TS21: palatal shelf Present 2N inset , 2N , 2M
Specimen 2O,P: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: palatal shelf Present 2P , 2P inset , 2O Number of expressing cells at level of levator veli palatini were comparable to that of control.
Specimen 3B: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl. Double labeled: green - Myod1; red - pH3.
Structure Level Pattern Image Note
TS21: palatal shelf Present 3B Few myogenic cells in control.
Specimen 3C: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl. Double labeled: green - Myod1; red - Casp3 (assay MGI:7488413).
Structure Level Pattern Image Note
TS21: palatal shelf Present 3C Few myogenic cells in control.
Specimen 3E: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - pH3.
Structure Level Pattern Image Note
TS21: palatal shelf Present 3E Few myogenic cells in mutant
Specimen 3F: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - Casp3 (assay MGI:7488413).
Structure Level Pattern Image Note
TS21: palatal shelf Present 3F Few myogenic cells in mutant
Specimen 4G,I: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl. Double labeled: green - Myod1; red - gamma-tubulin.
Structure Level Pattern Image Note
TS21: palatal shelf Present 4I , 4G
Specimen 4H,J: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - gamma-tubulin.
Structure Level Pattern Image Note
TS21: palatal shelf Present 4J , 4H
Specimen 4K,M: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl. Double labeled: green - Myod1; red - Arl13b (assay MGI:7488415).
Structure Level Pattern Image Note
TS21: palatal shelf Present 4K , 4M
Specimen 4L,N: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - Arl13b (assay MGI:7488415).
Structure Level Pattern Image Note
TS21: palatal shelf Present 4N , 4L
Specimen 4O,Q: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl. Double labeled: green - Myod1; red - Ttll3 (assay MGI:7488418).
Structure Level Pattern Image Note
TS21: palatal shelf Present 4Q , 4O
Specimen 4P,R: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - Ttll3 (assay MGI:7488418).
Structure Level Pattern Image Note
TS21: palatal shelf Present 4P , 4R
Specimen 7C,D: embryonic day 13.5; Ctnnb1tm2Kem/? (more )
Note: Controls were either Ctnnb1 fl/+ or fl/fl. Double labeled: green - Myod1; red - Dlk1 (assay MGI:7488423).
Structure Level Pattern Image Note
TS21: palatal shelf Present 7C , 7D
Specimen 7E,F: embryonic day 13.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Osr2tm5(cre)Jian/Osr2+ (more )
Note: Conditional mutant. Double labeled: green - Myod1; red - Dlk1 (assay MGI:7488423).
Structure Level Pattern Image Note
TS21: palatal shelf Present 7F , 7E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory