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Gene Expression Data
Assay Details
Assay
Reference: J:140337 Balastik M, et al., Deficiency in ubiquitin ligase TRIM2 causes accumulation of neurofilament light chain and neurodegeneration. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):12016-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7487984
Gene symbol: Nefl
Gene name: neurofilament, light polypeptide
Antibody: NR4
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5A: postnatal day 30 (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Present 5A
Specimen 5B: postnatal day 30; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Strong 5B Increased level of expression in cerebellar white matter compared to wild type.
TS28: cerebellum granule cell layer Present Regionally restricted 5B inset , 5B Expression in sporadic aggregates in granule cell layer.
Specimen 5C: postnatal day 62; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Note: Double labeled: red - Nefl; green - Calb1 (assay MGI:7487982).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell Present Regionally restricted 5C Expression in Purkinje cell axonal swellings co-localizing with Calb1 expression.
Specimen 5D: postnatal day 151 (more )
Structure Level Pattern Image Note
TS28: optic nerve Absent 5D
Specimen 5E: postnatal day 151; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Structure Level Pattern Image Note
TS28: optic nerve Present Regionally restricted 5E Expression in axonal swellings in optic nerve.
Specimen S8A: postnatal day 62 (more )
Structure Level Pattern Image Note
TS28: frontal cortex Present S8A
Specimen S8B: postnatal day 62; Trim2Gt(IRESBetageo)11Pgr/Trim2Gt(IRESBetageo)11Pgr (more )
Structure Level Pattern Image Note
TS28: frontal cortex Present Regionally restricted S8B Expression mostly in perikaryal inclusions in frontal cortex.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory