Gene Expression Data
Assay Details
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Assay
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Reference:
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J:97217
Wagner N, et al., A splice variant of the Wilms' tumour suppressor Wt1 is required for normal development of the olfactory system. Development. 2005 Mar;132(6):1327-36
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7471201
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Gene symbol:
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Wt1
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Gene name:
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WT1 transcription factor
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Antibody:
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6F-H2 (M3561)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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Assay notes:
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Two Wt1 antibodies were listed in the methods: MGI:3038122 and MGI:3027083.
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Results
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Specimen
not shown:
embryonic day 9.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1Aa:
embryonic day 18.5
(more )
Note: Double labeled: red - Wt1; green - Ncam1 (assay MGI:7481258).
Specimen
1Aa:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Wt1; green - Ncam1 (assay MGI:7481258).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: olfactory epithelium |
Present |
Regionally restricted |
1Ac
,
1Aa
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Cells with nuclear Wt1 staining were located predominantly in the basal region. A proportion of the Wt1-immunolabeled cells were also positive for Ncam1.
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Specimen
1Ba:
embryonic day 18.5
(more )
Note: Double labeled: red - Wt1; green - Gfap (assay MGI:7481260).
Specimen
1Ba:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Wt1; green - Gfap (assay MGI:7481260).
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Specimen
4Aa:
embryonic day 18.5
(more )
Specimen
4Aa:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: olfactory epithelium |
Present |
Regionally restricted |
4Aa
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Expression was detected in the basal part of the olfactory epithelium.
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Specimen
4Ab:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wt1tm2Asc/Wt1tm2Asc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: olfactory epithelium |
Present |
Regionally restricted |
4Ab
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Expression was detected in the basal part of the olfactory epithelium in the -KTS splice variant mutant.
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Specimen
4Ac:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wt1tm1Asc/Wt1tm1Asc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: olfactory epithelium |
Present |
Regionally restricted |
4Ac
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Expression was detected in the basal part of the olfactory epithelium in the +KTS splice variant mutant. This mutant had a thinner olfactory epithelium than in wild type.
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Specimen
8A:
embryonic day 18.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8C:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wt1tm2Asc/Wt1tm2Asc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8E:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wt1tm1Asc/Wt1tm1Asc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools