Gene Expression Data
Assay Details
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Assay
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Reference:
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J:311215
Fazilaty H, et al., Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage. Cell Rep. 2021 Aug 3;36(5):109484
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7461505
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
1A - E14.5:
(close  )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1A - E15.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1A - E16.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1A - E17.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1A - E18.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hindgut mesenchyme |
Present |
Non-Uniform |
1A E18.5
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Crypts are clearly discernible at this age. Higher expressing cells are near the top of the invaginations and lower expressing cells near the bottom.
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Specimen
1A - Adult:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3G:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pdgfra; magenta - Muc2 (assay MGI:7461523).
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Specimen
4C - E14.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Pdgfra; gray - Epcam (assay MGI:7461528); magenta - Apoa1 (assay MGI:7461521).
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Specimen
4C - E15.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Pdgfra; gray - Epcam (assay MGI:7461528); magenta - Apoa1 (assay MGI:7461521).
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Specimen
4C - E18.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Pdgfra; gray - Epcam (assay MGI:7461528); magenta - Apoa1 (assay MGI:7461521).
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Specimen
4C - Adult:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Pdgfra; gray - Epcam (assay MGI:7461528); magenta - Apoa1 (assay MGI:7461521).
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Specimen
S4B - E14.5:
embryonic day 14.5; Pdgfratm11(EGFP)Sor/Pdgfra +
(more  )
Note: Transverse section.
Specimen
S4B - E14.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Transverse section.
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Specimen
S4B - E15.5:
embryonic day 15.5; Pdgfratm11(EGFP)Sor/Pdgfra +
(more )
Note: Transverse section.
Specimen
S4B - E15.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Transverse section.
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Specimen
S4B - E18.5:
embryonic day 18.5; Pdgfratm11(EGFP)Sor/Pdgfra +
(more )
Note: Transverse section.
Specimen
S4B - E18.5:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Transverse section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hindgut mesenchyme |
Present |
Non-Uniform |
S4B E18.5
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Crypts are clearly discernible at this age. Higher expressing cells are near the top of the invaginations and lower expressing cells near the bottom.
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Specimen
S4C:
embryonic day 18.5; Pdgfratm11(EGFP)Sor/Pdgfra +
(more )
Note: Transverse section.
Specimen
S4C:
(close )
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Genetic Background:
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B6.129S4-Pdgfratm11(EGFP)Sor
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Transverse section.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: hindgut mesenchyme |
fibroblast |
Present |
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S4C
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Expression is seen in fibroblasts at the crypt top region.
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Analysis Tools