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Gene Expression Data
Assay Details
Assay
Reference: J:334115 Neirijnck Y, et al., Single-cell transcriptomic profiling redefines the origin and specification of early adrenogonadal progenitors. Cell Rep. 2023 Feb 28;42(3):112191
Assay type: RNA in situ
MGI Accession ID: MGI:7448477
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Probe: Mm-Nefm (315611)
Probe preparation: Antisense
Visualized with: Fluorescence
Results
Specimen 2G E9.0 Nefm: embryonic day 9.0 (more )
Note: Double labeled: blue - Nefm; red - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS15: urogenital ridge Ambiguous 2G E9.0 MERGE (upper) , 2G E9.0 Nefm , 2G E9.0 MERGE (lower) Expression not described.
Specimen 2G E10.5 anterior Nefm: embryonic day 10.5 (more )
Note: Double labeled: blue - Nefm; red - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS17: adrenogonadal primordium Present Regionally restricted 2G E10.5 anterior Nefm , 2G E10.5 anterior MERGE (upper) , 2G E10.5 anterior MERGE (lower) Expression in Nr5a1-expressing adrenal progenitors. Expression in some cells not expressing Nr5a in vicinity of adrenal primordium.
TS17: gonad primordium Absent 2G E10.5 anterior Nefm , 2G E10.5 anterior MERGE (upper) , 2G E10.5 anterior MERGE (lower)
Specimen 2G E10.5 posterior Nefm: embryonic day 10.5 (more )
Note: Double labeled: blue - Nefm; red - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS17: adrenogonadal primordium Present 2G E10.5 posterior MERGE (upper) , 2G E10.5 posterior MERGE (lower) , 2G E10.5 posterior Nefm
Specimen 2G E11.5 Nefm: embryonic day 11.5 (more )
Note: Double labeled: blue - Nefm; red - Nr5a1 (assay MGI:7448478).
Structure Level Pattern Image Note
TS19: adrenal primordium Present Regionally restricted 2G E11.5 Nefm , 2G E11.5 MERGE (upper) , 2G E11.5 MERGE (lower) Expression in Nr5a1-expressing adrenal cells. Expression in fetal adrenal cortex.
TS19: gonad Absent 2G E11.5 Nefm , 2G E11.5 MERGE (lower) , 2G E11.5 MERGE (upper)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory