Krt8 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:202344 Lesko MH, et al., Sox2 modulates the function of two distinct cell lineages in mouse skin. Dev Biol. 2013 Oct 1;382(1):15-26
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7447388
Gene symbol:	Krt8
Gene name:	keratin 8

Antibody:	TROMA 1
Detection system:	Secondary antibody

Results

5B ©

Specimen 5B: postnatal day 2 (more

)
Note: Double labeled: green - Krt8; red - Nefh (assay MGI:7447390).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	Merkel cell	Present		5B

5I ©

Specimen 5I: postnatal day 2 (more

)
Note: Double labeled: green - Krt8; red - Krt14 (assay MGI:7447381).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	Merkel cell	Present		5I

5J ©

Specimen 5J: embryonic day 19.5 (more

)
Note: Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		5J	Coexpressed with Sox2.

6A ©

Specimen 6A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6A

6B ©

Specimen 6B: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6B

6C ©

Specimen 6C: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6C	Some Merkel cells still differentiated.

6D ©

Specimen 6D: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6D	Some Merkel cells still differentiated.

6E ©

Specimen 6E: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6E

6F ©

Specimen 6F: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6F	Some Merkel cells still differentiated.

6G ©

Specimen 6G: postnatal day 21; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6G

6H ©

Specimen 6H: postnatal day 21; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6H

6I ©

Specimen 6I: postnatal day 21; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6I	Some Merkel cells still differentiated.

6J ©

Specimen 6J: postnatal day 21; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6J	Some Merkel cells still differentiated.

7A ©

Specimen 7A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		7A

7B ©

Specimen 7B: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		7B	Significant reduction in the number of positive Merkel cells per touch dome, compared with the control.

9A ©

Specimen 9A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9A

9E ©

Specimen 9E: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9E

9F ©

Specimen 9F: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9F

9G ©

Specimen 9G: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9G	Reduction in the number of positive Merkel cells compared with the control, although with a similar innervated Merkel cells per touch dome.

S1C ©

Specimen S1C: embryonic day 18.5 (more

)
Note: Triple labeled: green - Krt8; blue - Krt14 (assay MGI:7447401); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1C	Coexpressed with Sox2.

S1D ©

Specimen S1D: embryonic day 18.5 (more

)
Note: Triple labeled: green - Krt8; blue - Krt14 (assay MGI:7447401); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1D	Coexpressed with Sox2.

S1E ©

Specimen S1E: embryonic day 19.5 (more

)
Note: Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1E	Coexpressed with Sox2.

S1F ©

Specimen S1F: embryonic day 19.5 (more

)
Note: Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1F	Coexpressed with Sox2.

S2A ©

Specimen S2A: postnatal day 2 (more

)
Note: Double labeled: green - Krt8; red - Krt20 (assay MGI:7447402).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	Merkel cell	Present		S2A	Coexpressed with Krt20.

S2B ©

S2B" ©

Specimen S2B/B": embryonic day 18.5 (more

)
Note: Double labeled: green - Krt8; red - Ncam1 (assay MGI:7447403).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S2B" , S2B	Coexpressed with Ncam1 in some of the cells.

S3A ©

Specimen S3A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn (more

)
Note: Double labeled: green - Krt8; red - Mki67 (assay MGI:7447404).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3A

S3B ©

Specimen S3B: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Mki67 (assay MGI:7447404).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3B	Reduced number of positive Merkel cells, compared with the control.

S3E ©

Specimen S3E: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn (more

)
Note: Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Casp3 (assay MGI:7447405).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3E

S3F ©

Specimen S3F: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Casp3 (assay MGI:7447405).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3F	Reduced number of positive Merkel cells, compared with the control.

Specimen not shown: embryonic day 16.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: back skin	Merkel cell	Present
TS24: epidermis basal layer	Merkel cell	Present

Specimen not shown: embryonic day 16.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: epidermis basal layer	Merkel cell	Present
TS24: mystacial pad	Merkel cell	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23