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Gene Expression Data
Assay Details
Assay
Reference: J:215708 Osorio C, et al., Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci. 2014 Mar;59:24-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7444972
Gene symbol: Nefh
Gene name: neurofilament, heavy polypeptide
Antibody: Anti-Nefh (ab4680)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 2A middle: postnatal day 10 (more )
Note: Double labeled: red - Nefh; green - Tnfsf13 (assay MGI:7444967).
Structure Cell Type Level Pattern Image Note
TS28: hippocampus hippocampal pyramidal neuron Present Regionally restricted 2A Neurofilament middle , 2A Merge middle Expression was detected in the pyramidal cell axons.

Specimen 2A bottom: postnatal day 10 (more )
Note: Double labeled: red - Nefh; green - Tnfsf13 (assay MGI:7444967).
Structure Cell Type Level Pattern Image Note
TS28: fimbria hippocampus hippocampal pyramidal neuron Present Regionally restricted 2A Neurofilament bottom , 2A Merge bottom Expression was detected in the pyramidal cell axons.
TS28: hippocampus stratum oriens hippocampal pyramidal neuron Present Regionally restricted 2A Neurofilament bottom , 2A Merge bottom Expression was detected in the pyramidal cell axons.

Specimen 2B middle: postnatal day 10 (more )
Note: Double labeled: red - Nefh; green - Tnfrsf17 (assay MGI:7444968).
Structure Cell Type Level Pattern Image Note
TS28: hippocampus hippocampal pyramidal neuron Present Regionally restricted 2B Neurofilament middle , 2B Merge middle Expression was detected in the pyramidal cell axons.

Specimen 2B bottom: postnatal day 10 (more )
Note: Double labeled: red - Nefh; green - Tnfrsf17 (assay MGI:7444968).
Structure Cell Type Level Pattern Image Note
TS28: fimbria hippocampus hippocampal pyramidal neuron Present Regionally restricted 2B Merge bottom , 2B Neurofilament bottom Expression was detected in the pyramidal cell axons. Co-expression was noted with Tnfrsf17.
TS28: hippocampus stratum oriens hippocampal pyramidal neuron Present Regionally restricted 2B Merge bottom , 2B Neurofilament bottom Expression was detected in the pyramidal cell axons. Co-expression was noted with Tnfrsf17.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory