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Gene Expression Data
Assay Details
Assay
Reference: J:316179 Wang A, et al., An epigenetic circuit controls neurogenic programs during neocortex development. Development. 2021;148:dev199772
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7444000
Gene symbol: Eomes
Gene name: eomesodermin
Results
Specimen 2D TBR2 WT: postnatal adult (more )
Note: Double labeled: red - Tbr2 (Eomes); green - Pax6 (assay MGI:7443999).
Structure Cell Type Level Pattern Image Note
TS28: neocortex neural progenitor cell Present 2D WT PAX6/TBR2 , 2D WT TBR2 , 2D WT (enlarged) Expression in intermediate neural progenitor cells.
Specimen 2D TBR2 KO: postnatal adult; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Note: Double labeled: red - Tbr2 (Eomes); green - Pax6 (assay MGI:7443999).
Structure Cell Type Level Pattern Image Note
TS28: neocortex neural progenitor cell Strong Regionally restricted 2D KO PAX6/TBR2 , 2D KO (enlarged) , 2D KO TBR2 Increased number of expressing cells, with more co-expression with Pax6.
Specimen S3E TBR2 WT: embryonic day 16.5 (more )
Note: Double labeled: red - Tbr2 (Eomes); green - Pax6 (assay MGI:7443999).
Structure Cell Type Level Pattern Image Note
TS24: neocortex neural progenitor cell Present S3E WT (left) , S3E WT (right) Expression in intermediate neural progenitor cells.
Specimen S3E TBR2 KO: embryonic day 16.5; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Note: Double labeled: red - Tbr2 (Eomes); green - Pax6 (assay MGI:7443999).
Structure Cell Type Level Pattern Image Note
TS24: neocortex neural progenitor cell Strong Regionally restricted S3E KO (right) , S3E KO (left) Significantly increased number of expressing cells, with more co-expression with Pax6.
Specimen 2I TBR2 WT: embryonic day 13.5 (more )
Note: Double labeled: red - Tbr2 (Eomes); green - Pax6 (assay MGI:7443999).
Structure Cell Type Level Pattern Image Note
TS21: neocortex neural progenitor cell Present 2I WT Merge , 2I WT PAX6/TBR2 , 2I WT TBR2 Expression in intermediate neural progenitor cells.
Specimen 2I TBR2 KO: embryonic day 13.5; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Note: Double labeled: red - Tbr2 (Eomes); green - Pax6 (assay MGI:7443999).
Structure Cell Type Level Pattern Image Note
TS21: neocortex neural progenitor cell Present Regionally restricted 2I KO PAX6/TBR2 , 2I KO TBR2 , 2I KO Merge Increased co-expression with Pax6.
Specimen 3A TBR2 WT: embryonic day 16.5 (more )
Note: Triple labeled: red - Tbr2 (Eomes); blue/green - Mki67 (assay MGI:7444001); green - BrdU.
Structure Cell Type Level Pattern Image Note
TS24: neocortex neural progenitor cell Present 3A WT TBR2/BrdU/Ki67 (enlarged) , 3A WT TBR2/BrdU , 3A WT TBR2/BrdU/Ki67 (lower) , 3A WT TBR2/Ki67 , 3A WT TBR2/BrdU/Ki67 (upper) Expression in intermediate neural progenitor cells.
Specimen 3A TBR2 KO: embryonic day 16.5; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Note: Triple labeled: red - Tbr2 (Eomes); blue/green - Mki67 (assay MGI:7444001); green - BrdU.
Structure Cell Type Level Pattern Image Note
TS24: neocortex neural progenitor cell Weak Regionally restricted 3A KO TBR2/BrdU/Ki67 (lower) , 3A KO TBR2/BrdU/Ki67 (upper) , 3A KO TBR2/Ki67 , 3A KO TBR2/BrdU , 3A KO TBR2/BrdU/Ki67 (enlarged) Significantly fewer expressing cells. Larger proportion of expressing cells incorporating BrdU. Fewer triple-labeled cells.
Specimen 3G TBR2 WT: embryonic day 16.5 (more )
Note: Triple labeled: blue - Tbr2 (Eomes); red - Edu; green - BrdU.
Structure Cell Type Level Pattern Image Note
TS24: neocortex neural progenitor cell Present 3G WT (enlarged) , 3G WT (lower) , 3G WT BrdU/TBR2 , 3G WT (upper) Expression in intermediate neural progenitor cells.
Specimen 3G TBR2 KO: embryonic day 16.5; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Note: Triple labeled: blue - Tbr2 (Eomes); red - Edu; green - BrdU.
Structure Cell Type Level Pattern Image Note
TS24: neocortex neural progenitor cell Present Regionally restricted 3G KO (lower) , 3G KO (enlarged) , 3G KO (upper) , 3G KO BrdU/TBR2 Authors report intermediate progenitor cells showed longer cell-cycle time and longer S-phase period.
Specimen S4E TBR2 WT: embryonic day 13.5 (more )
Note: Triple labeled: red - Tbr2 (Eomes); blue/green - Mki67 (assay MGI:7444001); green - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: neocortex neural progenitor cell Present S4E WT TBR2/BrdU/Ki67 , S4E WT TBR2/BrdU , S4E WT TBR2/Ki67 , S4E WT Merge Expression in intermediate neural progenitor cells.
Specimen S4E TBR2 KO: embryonic day 13.5; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Note: Triple labeled: red - Tbr2 (Eomes); blue/green - Mki67 (assay MGI:7444001); green - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: neocortex neural progenitor cell Present Regionally restricted S4E KO TBR2/BrdU , S4E KO TBR2/Ki67 , S4E KO Merge , S4E KO TBR2/BrdU/Ki67 Signficantly more cells co-expression Mki67.
Specimen 4F TBR2 WT: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb neural progenitor cell Present 4F WT TBR2 Expression in intermediate neural progenitor cells.
Specimen 4F TBR2 KO: postnatal adult; Lncbarem1Bcgen/Lncbarem1Bcgen (more )
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb neural progenitor cell Weak 4F KO TBR2 Numbers of expressing cells lower than in wildtype.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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