Antibody:	anti-Aqp4
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS27: brain	Trace	Widespread	4A P1	Scarce expression in all brain regions.
TS27: cerebral cortex	Trace	Regionally restricted	4A P1	Cortical expression no longer restricted to ventral areas; expression begins to be marked laterally.

Structure	Level	Pattern	Image	Note
TS27: brain	Trace	Widespread	4A P3	Scarce expression in all brain regions.
TS27: cerebral cortex	Trace	Regionally restricted	4A P3	Cortical expression no longer restricted to ventral areas; expression begins to be marked laterally.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak	Regionally restricted	4A P7	Cortical expression expanded to lateral and dorsal regions. Expression weak but starts to organize around small vessels.
TS28: corpus callosum	Strong		4A P7	Intense expression along corpus callosum region.
TS28: lateral ventricle ependyma	Present		4A P7	Expression in ependymal cells bordering ventricle wall.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	4A P11	Expression pattern in cortex remains the same as at P7.
TS28: corpus callosum	Present		4A P11	Expression in corpus callosum lower in intensity than at P7.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	4A P20	Expression in vasculature stands out clearly in brain cortex.
TS28: corpus callosum	Present		4A P20	Expression in corpus callosum similar to that at P11.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Absent		4A P60	Expression no longer detected in corpus callosum.
TS28: cerebral cortex	Strong		4A P60
TS28: lateral ventricle ependyma	Strong		4A P60

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Absent		4A 18M	Expression no longer detected in corpus callosum.
TS28: cerebral cortex	Strong		4A 18M
TS28: lateral ventricle ependyma	Very strong		4A 18M	Expression in ependyma increases with age of animal.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Trace		5A P1	Cerebellum is very poorly developed and crest is barely recognizable; expression is very weak.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Trace		5A P3	Cerebellum is very poorly developed and crest is barely recognizable; expression is very weak.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	5A P7	High expression in white matter of cerebellum crest. Expression in deep part of cerebellar lobules, specifically in myelin-enriched internal branches.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	5A P11	Same pattern of expression as at P7, but weaker in intensity.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Weak		5A P20	Lower intenstiy of expression.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Trace		5A P60	Very low levels of expression throughout adult life.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Trace		5A 18M	Very low levels of expression throughout adult life.

Structure	Level	Pattern	Image	Note
TS27: spinal cord lumbar region	Present	Regionally restricted	5B P1	Expression limited to edges of spinal cord. Few differences detected between gray matter and white matter.

Structure	Level	Pattern	Image	Note
TS27: spinal cord lumbar region	Present	Regionally restricted	5B P3	Expression in white matter begins to be more intense than in gray matter zone.

Structure	Level	Pattern	Image	Note
TS28: spinal cord lumbar region	Present	Regionally restricted	5B P7	More intense peripheral expression coincident with external location of white matter.

Structure	Level	Pattern	Image	Note
TS28: spinal cord lumbar region	Present	Regionally restricted	5B P11	Expression in white matter decreases significanlygly, reaching low levels observed in gray matterl.

Structure	Level	Pattern	Image	Note
TS28: spinal cord lumbar region	Present	Regionally restricted	5B P20	Higher expression in gray matter in dorsal horns. Expression in white matter strongly reduced.

Structure	Level	Pattern	Image	Note
TS28: spinal cord lumbar region	Present	Regionally restricted	5B P60	Higher expression in gray matter in dorsal horns. Expression in white matter strongly reduced.

Structure	Level	Pattern	Image	Note
TS28: spinal cord lumbar region	Present	Regionally restricted	5B 18M	Higher expression in gray matter in dorsal horns. Expression in white matter strongly reduced.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus callosum	glial cell	Present	Regionally restricted	6A , 6A inset	Larger population of cells co-expressing Gfap than at P60. Expression in cells with less mature glial cell phenotype (increased size and more nonspecific distribution of expression in cell membrane).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus callosum	astrocyte	Present	Regionally restricted	6B , 6B inset	Expression mainly polarized at interface between astrocyte endfeet processes and vessels.