About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:37463 Farinas I, et al., Lack of neurotrophin-3 results in death of spinal sensory neurons and premature differentiation of their precursors. Neuron. 1996 Dec;17(6):1065-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7439188
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament 150
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Fixative was either 4%paraformaldehyde or Carnoy's fixative.
Results
Specimen 1D: embryonic day 10.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS17: thoracic dorsal root ganglion Present 1D Expression in this mutant is the same as in wild-type.
Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: thoracic dorsal root ganglion Present
Specimen 1I: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: L1 dorsal root ganglion Present 1I
Specimen 1J: embryonic day 13.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS21: L1 dorsal root ganglion Present 1J Expression indicates a reduction in ganglion size in this mutant compared to wild-type.
Specimen 5F: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present 5F
TS19: nerve Present 5F
Specimen 6A: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present 6A
TS19: neural tube Present 6A
Specimen 6B: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS19: peripheral nerve Present 6B
Specimen 6C: embryonic day 15.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS23: dorsal root ganglion Present 6C
Specimen 6D: embryonic day 13.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS21: peripheral nerve Present 6D
Specimen 6E: embryonic day 17.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS25: peripheral nerve Present 6E
Specimen 6G: embryonic day 17.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS25: peripheral nerve Present 6G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory