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Gene Expression Data
Assay Details
Assay
Reference: J:37463 Farinas I, et al., Lack of neurotrophin-3 results in death of spinal sensory neurons and premature differentiation of their precursors. Neuron. 1996 Dec;17(6):1065-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7439188
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament 150
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Fixative was either 4%paraformaldehyde or Carnoy's fixative.
Results
Specimen 1D: embryonic day 10.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS17: thoracic dorsal root ganglion Present 1D Expression in this mutant is the same as in wild-type.
Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: thoracic dorsal root ganglion Present
Specimen 1I: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: L1 dorsal root ganglion Present 1I
Specimen 1J: embryonic day 13.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS21: L1 dorsal root ganglion Present 1J Expression indicates a reduction in ganglion size in this mutant compared to wild-type.
Specimen 5F: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present 5F
TS19: nerve Present 5F
Specimen 6A: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present 6A
TS19: neural tube Present 6A
Specimen 6B: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS19: peripheral nerve Present 6B
Specimen 6C: embryonic day 15.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS23: dorsal root ganglion Present 6C
Specimen 6D: embryonic day 13.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS21: peripheral nerve Present 6D
Specimen 6E: embryonic day 17.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS25: peripheral nerve Present 6E
Specimen 6G: embryonic day 17.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: brown - Nefm; blue - Ntf3 reporter (assay MGI:7439190).
Structure Level Pattern Image Note
TS25: peripheral nerve Present 6G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory