Antibody:	anti-Blimp-1 (5E7; sc-130917)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	1A E7.5	Expression is seen in diploid trophoblasts and forming spiral artery-associated trophoblast giant cells (SpA-TGCs).

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present	Regionally restricted	1A E8.5	Expression is seen in diploid trophoblasts and forming spiral artery-associated trophoblast giant cells (SpA-TGCs).

Structure	Level	Pattern	Image	Note
TS15: spongiotrophoblast	Strong	Regionally restricted	1B , 1D , 1F	Expression is seen in spiral artery-associated trophoblast giant cells (SpA-TGCs) associated with maternal blood vessels; glycogen trophoblasts (Gly-Ts); and diploid cells.
TS15: secondary trophoblast giant cell	Absent		1B inset , 1B
TS15: labyrinthine zone	Absent		1E , 1B	This layer includes syncitiotrophoblasts (Syn-Ts) and sinusoidal trophoblast giant cells (S-TGCs); they lack expression.

Structure	Level	Pattern	Image	Note
TS15: labyrinthine zone	Absent		1C	The mutant placenta lacks expression.
TS15: spongiotrophoblast	Absent		1C	The mutant placenta lacks expression.
TS15: secondary trophoblast giant cell	Absent		1C , 1C inset

Structure	Level	Pattern	Image	Note
TS15: spongiotrophoblast	Present	Single cells	1G	Expression is seen in glycogen trophoblasts (Gly-Ts).

Structure	Level	Pattern	Image	Note
TS15: spongiotrophoblast	Present	Regionally restricted	1H , 1I	Prdm1 expression is seen in 10-20% of the diploid cells in spongiotrophoblast layer; they are dividing. Prdm1 expression is also seen in the spiral artery-associated trophoblast giant cells (SpA-TGCs); they are not dividing.
TS15: labyrinthine zone	Absent		1H

Structure	Level	Pattern	Image	Note
TS20: spongiotrophoblast	Present	Regionally restricted	S5C , S5A , S5_low mag	Expression is seen in spiral artery-associated trophoblast giant cells (SpA-TGCs) and canal-associated trophoblast giant cells (C-TGCs).
TS20: labyrinthine zone	Absent		S5_low mag , S5B

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Single cells	S5_low mag	Expression is seen in glycogen trophoblasts (Gly-Ts).

Structure	Level	Pattern	Image	Note
TS20: spongiotrophoblast	Present	Single cells	S5D	Expression is seen in glycogen trophoblasts (Gly-Ts).