Pax7 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:328577 Kitajima Y, et al., Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line. Skelet Muscle. 2018;8(1):26
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7428774
Gene symbol:	Pax7
Gene name:	paired box 7

Reporter:	YFP
Antibody:	anti-GFP
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

1C ©

Specimen 1C: embryonic day 10.5; Pax7^tm1Onoy/Pax7^tm1Onoy (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		1C

1D ©

Specimen 1D: embryonic day 10.5; Pax7^tm1Onoy/Pax7^tm1Onoy (more

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Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		1D

1E Sol ©

Specimen 1E Sol: postnatal adult; Pax7^tm1Onoy/Pax7^tm1Onoy (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS28: soleus	skeletal muscle satellite cell	Present		1E Sol

1E EDL ©

Specimen 1E EDL: postnatal adult; Pax7^tm1Onoy/Pax7^tm1Onoy (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS28: extensor digitorum longus	skeletal muscle satellite cell	Present		1E EDL

1F left ©

1F right ©

Specimen 1F: postnatal adult; Pax7^tm1Onoy/Pax7^tm1Onoy (more

)
Note: Double labeled: green - Pax7 reporter; red - Lama2 (assay MGI:7428801).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibialis anterior	skeletal muscle satellite cell	Present		1F left , 1F right

1G left ©

1G right ©

Specimen 1G: postnatal adult; Pax7^tm1Onoy/Pax7^tm1Onoy (more

)
Note: Double labeled: green - Pax7 reporter; red - Pax7 protein (assay MGI:7428807).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibialis anterior	skeletal muscle satellite cell	Present		1G right , 1G left	The YFP reporter was co-expressed with Pax7 in satellite cells in tibialis anterior.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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