Gene Expression Data
Assay Details
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Assay
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Reference:
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J:328577
Kitajima Y, et al., Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line. Skelet Muscle. 2018;8(1):26
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7428774
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Gene symbol:
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Pax7
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Gene name:
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paired box 7
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Reporter:
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YFP
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Antibody:
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anti-GFP
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1C:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Pax7tm1Onoy/Pax7tm1Onoy
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1D:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Pax7tm1Onoy/Pax7tm1Onoy
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1E Sol:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Pax7tm1Onoy/Pax7tm1Onoy
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: soleus |
skeletal muscle satellite cell |
Present |
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1E Sol
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Specimen
1E EDL:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Pax7tm1Onoy/Pax7tm1Onoy
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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Specimen
1F:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Pax7tm1Onoy/Pax7tm1Onoy
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax7 reporter; red - Lama2 (assay MGI:7428801).
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Specimen
1G:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Pax7tm1Onoy/Pax7tm1Onoy
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax7 reporter; red - Pax7 protein (assay MGI:7428807).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: tibialis anterior |
skeletal muscle satellite cell |
Present |
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1G right
,
1G left
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The YFP reporter was co-expressed with Pax7 in satellite cells in tibialis anterior.
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)
Analysis Tools