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Gene Expression Data
Assay Details
Assay
Reference: J:209131 Saba R, et al., CYP26B1 promotes male germ cell differentiation by suppressing STRA8-dependent meiotic and STRA8-independent mitotic pathways. Dev Biol. 2014 May 15;389(2):173-81
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7428692
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-KIT
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig 4G. Double labeled: green - Kit; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS23: germ cell of testis Trace 4C Most germ cells lost expression.
Specimen 4D: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig 4G. Double labeled: green - Kit; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 4D About 25% of the germ cells were positive.
Specimen 4E: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Data was quantified in Fig 4G. Double labeled: green - Kit; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 4E About 50% of the germ cells were positive.
Specimen 4F: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Triple labeled: green - Kit; magenta - Mki67 (assay MGI:7428698); blue - Gcna (assay MGI:7428697).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 4F About 50% of the germ cells were positive, which were also marked by Mki67.
Specimen 5D: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: green - Kit; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS24: germ cell of testis Ambiguous 5D Most of the germ cells were negative.
Specimen 5E: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: green - Kit; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 5E Expressed in some of the germ cells.
Specimen 5F: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Nanos2tm1Ysa/Nanos2tm1Ysa, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: green - Kit; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 5F Increased expression in the germ cells (about 89%).
Specimen not shown: embryonic day 13.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig S7B.
Structure Level Pattern Image Note
TS21: germ cell of testis Present About 15% of the germ cells were positive.
Specimen not shown: embryonic day 13.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig S7B.
Structure Level Pattern Image Note
TS21: germ cell of testis Present About 84% of the germ cells were positive.
Specimen not shown: embryonic day 13.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Data was quantified in Fig S7B.
Structure Level Pattern Image Note
TS21: germ cell of testis Present About 90% of the germ cells were positive.
Specimen not shown: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig S7B.
Structure Level Pattern Image Note
TS22: germ cell of testis Trace Most germ cells lost expression.
Specimen not shown: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig S7B.
Structure Level Pattern Image Note
TS22: germ cell of testis Present Expression declined compared with that of E13.5.
Specimen not shown: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Data was quantified in Fig S7B.
Structure Level Pattern Image Note
TS22: germ cell of testis Present Similar as that of E13.5.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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