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Gene Expression Data
Assay Details
Assay
Reference: J:209131 Saba R, et al., CYP26B1 promotes male germ cell differentiation by suppressing STRA8-dependent meiotic and STRA8-independent mitotic pathways. Dev Biol. 2014 May 15;389(2):173-81
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7428653
Gene symbol: Nanos2
Gene name: nanos C2HC-type zinc finger 2
Antibody: Nanos2 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig 2G. Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS23: germ cell of testis Strong Widespread 2A Expressed in all germ cells.
Specimen 2B: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig 2G. Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS23: germ cell of testis Ambiguous 2B Expression was completely suppressed in germ cells.
Specimen 2C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Data was quantified in Fig 2G. Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2C Only expressed in some of the germ cells (about 50%).
Specimen 5A: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: green - Nanos2; magenta - Kit (assay MGI:7428699).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 5A Expressed in some of the germ cells. Co-expressed with Kit was occasionally observed.
Specimen 5B: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: green - Nanos2; magenta - Kit (assay MGI:7428699).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 5B Expressed in some of the germ cells. Co-expressed with Kit was occasionally observed.
Specimen 5C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Triple labeled: green - Nanos2; blue - Gcna (assay MGI:7428697); magenta - Kit (assay MGI:7428699).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 5C Expressed in some of the germ cells. Co-expressed with Kit was occasionally observed.
Specimen S2A: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS22: germ cell of testis Present Widespread S2A Expressed in all germ cells.
Specimen S2B: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS22: germ cell of testis Absent S2B
Specimen S2C: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2C Only expressed in some of the germ cells.
Specimen S2D: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Data was quantified in Fig 2G. Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS26: germ cell of testis Strong S2D Expressed in all germ cells.
Specimen S2E: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Data was quantified in Fig 2G. Double labeled: green - Nanos2; magenta - Gcna (assay MGI:7428651).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S2E Only expressed in some of the germ cells (about 50%).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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