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Gene Expression Data
Assay Details
Assay
Reference: J:320419 Li R, et al., E3 ubiquitin ligase FBXW7 balances airway cell fates. Dev Biol. 2022 Mar;483:89-97
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427631
Gene symbol: Krt5
Gene name: keratin 5
Antibody: anti-Keratin 5 Polyclonal (#905501)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2I: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 2I
Specimen 2J: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 2J Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Specimen 4A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Notch1tm1Agt/Notch1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 4A
Specimen 4B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Notch1tm1Agt/Notch1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 4B Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Specimen 4C: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Notch1tm1Agt/Notch1tm1Agt, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 4C Ectopic development of luminal Trp63-Krt5+ cells observed in the Fbxw7 single mutant was reversed.
Specimen 5A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Klf5tm1Jaw/Klf5+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 5A
Specimen 5B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Klf5tm1Jaw/Klf5+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 5B Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Specimen 5C: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Klf5tm1Jaw/Klf5tm1Jaw, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present 5C Ectopic development of luminal Trp63-Krt5+ cells was reversed.
Specimen S4A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Shhtm1(EGFP/cre)Cjt/Shh+, Tgif1tm1Caw/Tgif1+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S4A Expressed in the basal layer.
Specimen S4B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+, Tgif1tm1Caw/Tgif1+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S4B Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Specimen S4C: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+, Tgif1tm1Caw/Tgif1tm1Caw (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S4C Ectopic development of luminal Trp63-Krt5+ cells persisted, similar to the Fbxw7 single mutant.
Specimen S5A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Notch1tm1Agt/Notch1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S5A
Specimen S5B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Notch1tm1Agt/Notch1tm1Agt, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S5B Similar as in the control.
Specimen S6A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Klf5tm1Jaw/Klf5+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S6A
Specimen S6B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Klf5tm1Jaw/Klf5tm1Jaw, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Krt5; red - Trp63 (assay MGI:7427630).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium basal cell of epithelium of trachea Present S6B Decreased expression compared with the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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