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Gene Expression Data
Assay Details
Assay
Reference: J:319706 Silachev D, et al., Mouse models characterize GNAO1 encephalopathy as a neurodevelopmental disorder leading to motor anomalies: from a severe G203R to a milder C215Y mutation. Acta Neuropathol Commun. 2022 Jan 28;10(1):9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7425428
Gene symbol: Pou3f2
Gene name: POU domain, class 3, transcription factor 2
Antibody: anti-Brn2 (sc-393324)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S6C +/+: embryonic day 18.5 (more )
Note: Double labeled: green - Pou3f2; red - Reln (assay MGI:7425422).
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C +/+ Brn2/Reelin Immunopositive cells were detected in layers II/III of the motor cortex.
Specimen S6C G203R/+: embryonic day 18.5; Gnao1em1Katv/Gnao1+ (more )
Note: Double labeled: green - Pou3f2; red - Reln (assay MGI:7425422).
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C G203R/+ Brn2/Reelin The number of immunopositive cells detected in layers II/III of the motor cortex was increased compared with the homozygous mutant and the wild type.
Specimen S6C G203R/G203R: embryonic day 18.5; Gnao1em1Katv/Gnao1em1Katv (more )
Note: Double labeled: green - Pou3f2; red - Reln (assay MGI:7425422).
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C G203R/G203R Brn2/Reelin Immunopositive cells were detected in layers II/III of the motor cortex.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory