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Gene Expression Data
Assay Details
Assay
Reference: J:331055 Chousal JN, et al., Progression of the pluripotent epiblast depends upon the NMD factor UPF2. Development. 2022 Nov 1;149(21):dev200764
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7425203
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog (eBioMLC-51, 14-5761-80)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5A +/+: embryonic day 3.5 (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 5A-1-2 , 5A-1-1 Expressed in epiblast precursor cells.
Specimen 5A +/-: embryonic day 3.5; Upf2tm1.1Btp/Upf2+ (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 5A-2-2 , 5A-2-1 Expressed in epiblast precursor cells. No significant difference in the number of positive cells compared with the wild-type.
Specimen 5A -/-: embryonic day 3.5; Upf2tm1.1Btp/Upf2tm1.1Btp (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 5A-3-2 , 5A-3-1 Expressed in epiblast precursor cells. Significantly lower number of positive cells compared with the wild-type.
Specimen S4A +/+: embryonic day 3.5 (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4A-1-1 , S4A-1-2 Expressed in epiblast precursor cells.
Specimen S4A +/-: embryonic day 3.5; Upf2tm1.1Btp/Upf2+ (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4A-2-2 , S4A-2-1 Expressed in epiblast precursor cells. No significant difference in the number of positive cells compared with the wild-type.
Specimen S4A -/- 1: embryonic day 3.5; Upf2tm1.1Btp/Upf2tm1.1Btp (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4A-3-2 , S4A-3-1 Expressed in epiblast precursor cells. Significantly lower number of positive cells compared with the wild-type.
Specimen S4A -/- 2: embryonic day 3.5; Upf2tm1.1Btp/Upf2tm1.1Btp (more )
Note: Triple labeled: green - Nanog; red - Gata6 (assay MGI:7425204); magenta - Cdx2 (assay MGI:7425202).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted S4A-4-2 , S4A-4-1 Expressed in epiblast precursor cells. Significantly lower number of positive cells compared with the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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