Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104307
Wilson ME, et al., The HMG Box Transcription Factor Sox4 Contributes to the Development of the Endocrine Pancreas. Diabetes. 2005 Dec;54(12):3402-9
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7408288
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 2% paraformaldehyde and 0.1% glutaraldehyde and post-fixed in 4% paraformaldehyde.
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Results
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Specimen
2A:
embryonic day 9.5; Sox2tm1Rlb/Sox2 +
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Sox2tm1Rlb/Sox2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2B:
embryonic day 9.5; Sox2tm1Rlb/Sox2 +
(more )
Note: Double labeled: blue - Sox2 reporter; brown - Pdx1 (assay MGI:7408289).
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Sox2tm1Rlb/Sox2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Sox2 reporter; brown - Pdx1 (assay MGI:7408289).
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Specimen
not shown E10.5:
embryonic day 10.5; Sox2tm1Rlb/Sox2 +
(more )
Specimen
not shown E10.5:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Sox2tm1Rlb/Sox2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
not shown E16.5:
embryonic day 16.5; Sox2tm1Rlb/Sox2 +
(more )
Specimen
not shown E16.5:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Sox2tm1Rlb/Sox2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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