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Gene Expression Data
Assay Details
Assay
Reference: J:186470 Lopes C, et al., Tlx3 and runx1 act in combination to coordinate the development of a cohort of nociceptors, thermoceptors, and pruriceptors. J Neurosci. 2012 Jul 11;32(28):9706-15
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388619
Gene symbol: Calca
Gene name: calcitonin/calcitonin-related polypeptide, alpha
Antibody: anti-CGRP
Results
Specimen 1C: postnatal day 30 (more )
Note: Double labeled: red - CGRP (Calca); green - Tlx3 (assay MGI:7388617).
Structure Cell Type Level Pattern Image Note
TS28: lumbar dorsal root ganglion neuron Present Regionally restricted 1C Expression in peptidergic neurons.
Specimen 3C Control: postnatal day 30; Tlx3tm2.1Qima/? (more )
Note: Littermate control; genotype either fl/fl or fl/+. Double labeled: green - CGRP (Calca); red - IB4.
Structure Cell Type Level Pattern Image Note
TS28: lumbar dorsal root ganglion neuron Weak Regionally restricted 3C Control Expression in 7.7% of IB4-positive neurons.
Specimen 3C Tlx3F/F;Nav1.8Cre/+: postnatal day 30; Tlx3tm2.1Qima/Tlx3tm2.1Qima, Tg(Scn10a-cre)1Rkun/0 (more )
Note: Conditional mutant. Double labeled: green - CGRP (Calca); red - IB4.
Structure Cell Type Level Pattern Image Note
TS28: lumbar dorsal root ganglion neuron Present Regionally restricted 3C Tlx3F/F;Nav1.8Cre/+ Expression in 22% of IB4-positive neurons.
Specimen 3D Control: postnatal day 30; Tlx3tm2.1Qima/? (more )
Note: Littermate control; genotype either fl/fl or fl/+. Double labeled: green - CGRP (Calca); red - IB4.
Structure Level Pattern Image Note
TS28: spinal cord Present Regionally restricted 3D Control Expression in sensory afferents mainly in lamina I and outer layer of lamina II.
Specimen 3D Tlx3F/F;Nav1.8Cre/+: postnatal day 30; Tlx3tm2.1Qima/Tlx3tm2.1Qima, Tg(Scn10a-cre)1Rkun/0 (more )
Note: Conditional mutant. Double labeled: green - CGRP (Calca); red - IB4.
Structure Level Pattern Image Note
TS28: spinal cord Present Regionally restricted 3D Tlx3F/F;Nav1.8Cre/+ Lamina-specific innervation largely unchanged compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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