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Gene Expression Data
Assay Details
Assay
Reference: J:329049 Galdos FX, et al., devCellPy is a machine learning-enabled pipeline for automated annotation of complex multilayered single-cell transcriptomic data. Nat Commun. 2022 Sep 7;13(1):5271
Assay type: RNA in situ
MGI Accession ID: MGI:7388330
Gene symbol: Tbx5
Gene name: T-box 5
Probe: Mm-Tbx5 (519581)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay. A minimum of three biological (different hearts) and three technical (different sections/heart) were used for each in situ hybridization.
Results
Specimen 5C - E10.5: embryonic day 10.5 (more )
Note: Double labeled: red - Tbx5; cyan - Ppp1r17 (assay MGI:7388329). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: primitive ventricle Present Regionally restricted 5C E10.5 merge , 5C E10.5 Tbx5 Expression is seen in the left ventricle.
Specimen 5C - E16.5: embryonic day 16.5 (more )
Note: Double labeled: red - Tbx5; cyan - Ppp1r17 (assay MGI:7388329). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS24: left atrium Present 5C E16.5 Tbx5 , 5C E16.5 merge
TS24: right atrium Present 5C E16.5 Tbx5 , 5C E16.5 merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory