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Gene Expression Data
Assay Details
Assay
Reference: J:266543 Zhang YL, et al., DCAF13 promotes pluripotency by negatively regulating SUV39H1 stability during early embryonic development. EMBO J. 2018 Sep 14;37(18)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388141
Gene symbol: Dcaf13
Gene name: DDB1 and CUL4 associated factor 13
Results
Specimen 1C Dcaf13 GV: postnatal adult (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS28: primary oocyte Absent 1C GV (merged) , 1C GV DCAF13
Specimen 1C Dcaf13 MII: postnatal adult (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS28: secondary oocyte Absent 1C MII (merged) , 1C MII DCAF13
Specimen 1C Dcaf13 1-cell: embryonic day 0.5 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent 1C 1-cell DCAF13 , 1C 1-cell (merged)
Specimen 1C Dcaf13 2-cell: embryonic day 1.0 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 1C 2-cell (merged) , 1C 2-cell DCAF13
Specimen 1C Dcaf13 4-cell: embryonic day 2.0 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Weak 1C 4-cell (merged) , 1C 4-cell DCAF13 Expression at 4-cell stage.
Specimen 1C Dcaf13 8-cell: embryonic day 2.5 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 1C 8-cell DCAF13 , 1C 8-cell (merged) Mainly localized in nucleoli of blastomeres.
Specimen 1C Dcaf13 morula: embryonic day 3.0 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS4: compacted morula Strong Regionally restricted 1C morula (merged) , 1C morula DCAF13 Accumulates in morula and blastocysts, mainly in nucleoli of blastomeres.
Specimen 1C Dcaf13 blastocyst: embryonic day 3.5 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS4: blastocyst Strong Regionally restricted 1C blastocyst (merged) , 1C blastocyst DCAF13 Accumulates in morula and blastocysts, mainly in nucleoli of blastomeres.
Specimen EV1C (upper) DCAF13: embryonic day 4.5 (more )
Note: Double labeled: green - Dcaf13; red - Cdx2 (assay MGI:7388145).
Structure Level Pattern Image Note
TS6: epiblast Present Uniform EV1C DAPI/DCAF13/CDX2 (upper) , EV1C DCAF13/CDX2 (upper) , EV1C DCAF13 (upper) Uniform expression in inner cell mass and trophectoderm cells.
TS6: trophectoderm Present Uniform EV1C DAPI/DCAF13/CDX2 (upper) , EV1C DCAF13/CDX2 (upper) , EV1C DCAF13 (upper) Uniform expression in inner cell mass and trophectoderm cells.
Specimen EV1C (lower) DCAF13: embryonic day 4.5 (more )
Note: Double labeled: green - Dcaf13; red - Cdx2 (assay MGI:7388145).
Structure Level Pattern Image Note
TS6: trophectoderm Present Uniform EV1C DAPI/DCAF13/CDX2 (lower) , EV1C DCAF13/CDX2 (lower) , EV1C DCAF13 (lower)
Specimen 2B E2.5 WT DCAF13: embryonic day 2.5 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B E2.5 WT DCAF13 , 2B E2.5 WT Merged
Specimen 2B E2.5 Dcaf13-/- DCAF13: embryonic day 2.5; Dcaf13em1Hyfn/Dcaf13em1Hyfn (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2B E2.5 Dcaf13-/- DCAF13 , 2B E2.5 Dcaf13-/- Merged Morphologically normal up to 8-cell stage.
Specimen 2B E3.5 WT DCAF13: embryonic day 3.5 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS4: blastocyst Present 2B E3.5 WT DCAF13 , 2B E3.5 WT Merged
Specimen 2B E3.5 Dcaf13-/- DCAF13: embryonic day 3.5; Dcaf13em1Hyfn/Dcaf13em1Hyfn (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS4: embryo Absent 2B E3.5 Dcaf13-/- DCAF13 , 2B E3.5 Dcaf13-/- Merged Embryos failed to develop into blastocysts.
Specimen 2B E4.5 WT DCAF13: embryonic day 4.5 (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS6: embryo Present 2B E4.5 WT Merged , 2B E4.5 WT DCAF13
Specimen 2B E4.5 Dcaf13-/- DCAF13: embryonic day 4.5; Dcaf13em1Hyfn/Dcaf13em1Hyfn (more )
Note: Double labeled: green - Dcaf13; red - B23 (Npm1 assay MGI:7388143).
Structure Level Pattern Image Note
TS6: embryo Absent 2B E4.5 Dcaf13-/- Merged , 2B E4.5 Dcaf13-/- DCAf13
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory