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Gene Expression Data
Assay Details
Assay
Reference: J:314181 Holzman MA, et al., HOXA5 Participates in Brown Adipose Tissue and Epaxial Skeletal Muscle Patterning and in Brown Adipocyte Differentiation. Front Cell Dev Biol. 2021;9:632303
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7384936
Gene symbol: Ebf2
Gene name: early B cell factor 2
Antibody: anti-EBF2 (AF7006)
Detection system: Secondary antibody
Results
Specimen 1C/D: embryonic day 14.5 (more )
Note: Double labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935).
Structure Cell Type Level Pattern Image Note
TS22: brown fat brown adipocyte Present Regionally restricted 1C , 1D Expression in adipocyte nuclei, colocalized with Hoxa5 expression.
Specimen 2C/D: embryonic day 12.5 (more )
Note: Triple labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935); white - Myh4 (assay MGI:7384937).
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 2C , 2D Expression in mesenchyme, mostly somite derived, flanking myotome extending laterally to scapula and medially to neural tube, colocalized with Hoxa5 expression.
Specimen 2G/H: embryonic day 12.5 (more )
Note: Double labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935).
Structure Cell Type Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 2H , 2G Expression in mesenchyme flanking epaxial myotome, colocalized with Hoxa5 expression.
TS20: myotome skeletal muscle fibroblast Present Regionally restricted 2G , 2H Expression in prospective muscle connective tissue fibroblasts interspersed within myotome.
TS20: skeletal muscle Absent 2G , 2H No expression in epaxial muscle mass.
Specimen S4T: embryonic day 16.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935).
Structure Level Pattern Image Note
TS24: trunk connective tissue Present Regionally restricted S4T
Specimen S4V/W: embryonic day 16.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935).
Structure Level Pattern Image Note
TS24: brown fat Present S4W , S4V
Specimen S4Y/Z: embryonic day 16.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935).
Structure Level Pattern Image Note
TS24: skeletal muscle Present S4Z , S4Y
Specimen S4AB: embryonic day 16.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Ebf2; green - Hoxa5 (assay MGI:7384935).
Structure Level Pattern Image Note
TS24: trachea Ambiguous S4AB
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory