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Gene Expression Data
Assay Details
Assay
Reference: J:316543 Kim JS, et al., Reciprocal localization of transcription factors YY1 and CP2c in spermatogonial stem cells and their putative roles during spermatogenesis. Acta Histochem. 2016 Sep;118(7):685-692
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7384820
Gene symbol: Tfcp2
Gene name: transcription factor CP2
Antibody: Anti-LSF (clone 14; 610818)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 4A1: postnatal week 12 (more )
Note: Two different stages of the seminiferous tubule - Elongation and Release stages. Double labeled: red - Tfcp2; green - Yy1 (assay MGI:7384819).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A1 Merge , 4A1 CP2c Yy1 and Tfcp2 localized in the nucleus and cytoplasm of spermatocytes, respectively.
Specimen 4A2: postnatal week 12 (more )
Note: Two different stages of the seminiferous tubule - Elongation and Release stages. Double labeled: red - Tfcp2; green - Yy1 (assay MGI:7384819).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A2 CP2c , 4A2 Merge Yy1 and Tfcp2 localized in the nucleus and cytoplasm of spermatocytes, respectively.
Specimen 4B: postnatal week 12 (more )
Note: Grouping stage. Double labeled: red - Tfcp2; green - Yy1 (assay MGI:7384819).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 4B Merge , 4B CP2c Nuclear co-localization of Yy1 and Tfcp2 in spermatogonia at Grouping stage seminiferous tubule.
Specimen 4C: postnatal week 12 (more )
Note: Grouping and Release stages. Double labeled: red - Tfcp2; green - Yy1 (assay MGI:7384819).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 4C' CP2c , 4C CP2c , 4C Merge , 4C'' CP2c , 4C' Merge , 4C'' Merge Co-localization of Yy1 and Tfcp2 in spermatogonia. Tfcp2 was mainly localized in the cytoplasm, although some of it was translocated into the nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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