Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316543
Kim JS, et al., Reciprocal localization of transcription factors YY1 and CP2c in spermatogonial stem cells and their putative roles during spermatogenesis. Acta Histochem. 2016 Sep;118(7):685-692
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7384677
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Gene symbol:
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Tfcp2
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Gene name:
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transcription factor CP2
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Results
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Specimen
1D:
embryonic day 14.5
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
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Present |
Regionally restricted |
1D'
,
1D
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Staining identified 30% of gonocytes were immunopositive.
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| TS22: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
1D
,
1D'
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Staining identified 18% of Sertoli cells were immunopositive.
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| TS22: testis mesenchyme |
mesenchymal cell |
Strong |
Non-Uniform |
1D
,
1D'
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Mesenchymal cells surrounding the testicular cords were strongly stained in the nuclei and faintly stained in the cytoplasm.
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Specimen
2B:
postnatal week 12
(more )
Specimen
2B:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal week 12
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
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Present |
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2B
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Expression was localized to the cytoplasm.
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| TS28: spermatogonium |
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Present |
Regionally restricted |
2B
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Some spermatogonia were immunolabeled. Expression was in both nucleus and cytoplasm of spermatogonia in specific stage of seminiferous tubules.
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| TS28: spermatid |
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Weak |
Regionally restricted |
2B
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A few spermatids were immunolabeled. Faint staining was noted in the cytoplasm.
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| TS28: testis |
male germ line stem cell |
Absent |
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2B
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Specimen
2C:
postnatal week 12
(more )
Note: Double labeled: brown - Tfcp2; blue - Yy1 (assay MGI:7384675).
Specimen
2C:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal week 12
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Tfcp2; blue - Yy1 (assay MGI:7384675).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
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Present |
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2C
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Expression was localized to the cytoplasm.
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| TS28: spermatogonium |
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Present |
Regionally restricted |
2C
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Some spermatogonia were immunolabeled. Expression was in both nucleus and cytoplasm of spermatogonia in specific stage of seminiferous tubules.
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| TS28: spermatid |
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Weak |
Regionally restricted |
2C
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A few spermatids were immunolabeled. Faint staining was noted in the cytoplasm.
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| TS28: testis |
male germ line stem cell |
Absent |
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2C
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Analysis Tools