Gene Expression Data
Assay Details
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Assay
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Reference:
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J:186260
Yoshida A, et al., Localization of septin proteins in the mouse cochlea. Hear Res. 2012 Jul;289(1-2):40-51
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7380403
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Gene symbol:
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Septin5
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Gene name:
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septin 5
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Results
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Specimen
4a/c:
postnatal adult
(more  )
Note: Double labeled: green - Septin5; red - Syp (assay MGI:7380405).
Specimen
4a/c:
(close  )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin5; red - Syp (assay MGI:7380405).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
external pillar cell of cochlea |
Strong |
|
4c
,
4c inset
,
4a
,
4a inset
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| TS28: organ of Corti |
internal pillar cell of cochlea |
Strong |
|
4c
,
4c inset
,
4a
,
4a inset
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| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
4c
,
4a
,
4c inset
,
4a inset
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Expression in filament-like structure arising from basilar membrane lateral to outer pillar cells.
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| TS28: cochlea |
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Present |
Regionally restricted |
4c
,
4c inset
,
4a inset
,
4a
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Expression overlapped with that of synaptophysin, a component of presynaptic vesicles of efferent nerve terminals.
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Specimen
4d:
postnatal adult
(more )
Note: Double labeled: green - Septin5; red - F-actin
Specimen
4d:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin5; red - F-actin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
4d
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Expression in punctate structure beneath inner hair cells and around basal portion of outer hair cells.
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Specimen
4e:
postnatal adult
(more )
Note: Double labeled: green - Septin5; red - Tubb3 (assay MGI:7380402).
Specimen
4e:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin5; red - Tubb3 (assay MGI:7380402).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Present |
Regionally restricted |
4e
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Expression in punctate structures below inner hair cells overlapped with that of Tubb3, suggesting expressiion in efferent nerve terminal of lateral and medial olivo-cochlear tract.
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Specimen
4f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Septin5tm1Wtr/Septin5tm1Wtr
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4g/i:
postnatal adult
(more )
Note: Double labeled: green - Septin5; red - acetylated tubulin.
Specimen
4g/i:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Double labeled: green - Septin5; red - acetylated tubulin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
4i
,
4g
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Expression overlapped with that of acetylated alpha tubulin in filament-like structure arising from basilar membrane.
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Specimen
6b/e:
postnatal adult
(more )
Note: Double labeled: green - Septin5; red - Myo7a (assay MGI:7380409).
Specimen
6b/e:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Septin5; red - Myo7a (assay MGI:7380409).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
6e
,
6b
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Expression in filament-like structures originating from basilar membrane and extending to basal portion of outer hair cells.
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Specimen
7a/c:
postnatal adult
(more )
Specimen
7a/c:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
7a
,
7c
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No expression in phalangeal processes of Deiters' cells.
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Specimen
7b/d:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Septin4tm1Ksh/Septin4tm1Ksh
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
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Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
7b
,
7d
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Expression in filament-like structure (phalangeal process) in Deiters' cells extending to apices of outer hair cells.
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Specimen
E13 not shown:
embryonic day 13.0
(more )
Specimen
E13 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cochlea |
Absent |
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No expression in cochlea until P3.
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Specimen
E15 not shown:
embryonic day 15.0
(more )
Specimen
E15 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Absent |
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No expression in cochlea until P3.
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Specimen
P1 not shown:
postnatal day 1
(more )
Specimen
P1 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Absent |
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No expression in cochlea until P3.
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Specimen
P2 not shown:
postnatal day 2
(more )
Specimen
P2 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Absent |
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No expression in cochlea until P3.
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Specimen
9g/i:
postnatal day 3
(more )
Note: Double labeled: green - Septin5; red - F-actin.
Specimen
9g/i:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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optical section
|
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin5; red - F-actin.
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Specimen
9j/l:
postnatal day 7
(more )
Note: Double labeled: green - Septin5; red - F-actin.
Specimen
9j/l:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin5; red - F-actin.
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Analysis Tools