Antibody:	anti-Calbindin (D1I4Q) (13176)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present	Regionally restricted	1A

Structure	Level	Pattern	Image	Note
TS24: greater epithelial ridge	Present	Regionally restricted	1B	Expression in greater epithelial ridge localized in cytosol of columnar epithelial cells. No co-expression with Sox2.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: greater epithelial ridge		Present		1C
TS24: organ of Corti	cochlear inner hair cell	Present		1C
TS24: cochlea	interdental cell of cochlea	Absent		1C	Expression in interdental cells in inner sulcus.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: organ of Corti	cochlear inner hair cell	Present		1D	Expression in cytosol of inner hair cells.
TS24: organ of Corti	cochlear outer hair cell	Absent		1D
TS24: organ of Corti	Deiter's cell	Absent		1D

Structure	Cell Type	Level	Pattern	Image	Note
TS24: greater epithelial ridge		Present	Regionally restricted	1E
TS24: cochlear ganglion	spiral ganglion neuron	Absent		1E
TS24: vestibulocochlear nerve cochlear component		Absent		1E	No expression in glial cells of Rosenthal's canal.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: greater epithelial ridge		Present	Regionally restricted	1F
TS26: organ of Corti	cochlear inner hair cell	Absent		1F

Structure	Cell Type	Level	Pattern	Image	Note
TS26: greater epithelial ridge		Present	Regionally restricted	1G
TS26: organ of Corti	cochlear inner hair cell	Present		1G
TS26: organ of Corti	cochlear outer hair cell	Absent		1G
TS26: cochlea	interdental cell of cochlea	Present		1G

Structure	Cell Type	Level	Pattern	Image	Note
TS26: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	1H	Expression throughout inner hair cell cytosol, including basal and apical regions. No co-localization with Myo7a.
TS26: organ of Corti	cochlear outer hair cell	Absent		1H

Structure	Cell Type	Level	Pattern	Image	Note
TS26: organ of Corti	cochlear inner hair cell	Present		1I
TS26: organ of Corti	cochlear outer hair cell	Absent		1I
TS26: organ of Corti	Deiter's cell	Absent		1I

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear inner hair cell	Present		2A	Expression within cytoplasm and nucleus.
TS27: organ of Corti	cochlear outer hair cell	Present		2A	Expression in 3 rows of outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: greater epithelial ridge		Present	Regionally restricted	2B
TS27: organ of Corti	organ of Corti supporting cell	Absent		2B	No expression in a few columnar inner supporting cells located medial to inner hair cells.
TS27: cochlea	interdental cell of cochlea	Present		2B	Expression maintained in interdental cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	2C	Expression in organ of Corti in both cytoplasm and nucleus of inner cells and outer hair cells, co-expressed with Myo7a
TS27: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	2C	Expression in organ of Corti in both cytoplasm and nucleus of inner cells and outer hair cells, co-expressed with Myo7a

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	2D , 2F	Expression in organ of Corti. Co-localization with phalloidin in cuticular plates of hair cells; no expression in stereociliary bundle of hair cells.
TS27: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	2D , 2F	Expression in organ of Corti. Co-localization with phalloidin in cuticular plates of hair cells; no expression in stereociliary bundle of hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear ganglion	spiral ganglion neuron	Present	Scattered	2I , 2G	Expression in cytoplasm and nucleus of subpopulation of scattered spinal ganglion neurons. Co-expression with Tubb3 in in cytoplasm.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	3A

Structure	Level	Pattern	Image	Note
TS28: greater epithelial ridge	Present		3B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	3C	Co-expressed with phalloidin in cuticular plates of hair cells. No expression in hair cell nuclei.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	3C	Co-expressed with phalloidin in cuticular plates of hair cells. No expression in hair cell nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlear ganglion	spiral ganglion neuron	Present	Regionally restricted	3D , 3F	Expression in spinal ganglion neuron (SGN) somata, mostly co-expressed with Tubb3, and in nucleus of SGN not expressing Tubb3.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	interdental cell of cochlea	Absent		3G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: greater epithelial ridge		Present	Regionally restricted	3H	Expression restricted to apical and basal parts of greater epithelial ridge.
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	3H	Expression co-localized with phalloidin in cuticular plates of hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	3H	Expression co-localized with phalloidin in cuticular plates of hair cells.
TS28: cochlea		Present	Regionally restricted	3H	Expression in inner sulcus.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlear ganglion	spiral ganglion neuron	Present	Regionally restricted	3I	Expression in spinal ganglion neuron somata and nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	4C , 4A	Expression co-localized with phalloidin in cuticular plates of hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	4C , 4A	Expression co-localized with phalloidin in cuticular plates of hair cells.
TS28: greater epithelial ridge		Trace	Regionally restricted	4C , 4A	Expression limited to apical surface of residual greater epithelial ridge cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	4F , 4D	Expression co-localized with phalloidin in cuticular plates of hair cells. No expression in stereociliary bundles.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	4F , 4D	Expression co-localized with phalloidin in cuticular plates of hair cells. No expression in stereociliary bundles.
TS28: greater epithelial ridge		Absent		4F , 4D	Expression completely disappeared from greater epithelial ridge.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	5A , 5C	Co-expression with calmodulin in cytoplasm of hair cells. No expression in stereociliary bundles.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	5A , 5C	Co-expression with calmodulin in cytoplasm of hair cells. No expression in stereociliary bundles.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	5D , 5F	Co-expression with calmodulin in cytoplasm of hair cells. No expression in stereociliary bundles.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	5D , 5F	Co-expression with calmodulin in cytoplasm of hair cells. No expression in stereociliary bundles.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	6C , 6A	Co-expression with Myo7a in cytoplasm of hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6C , 6A	Co-expression with Myo7a in cytoplasm of hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	6F , 6D	Co-expression with phaloidin in cuticular plates of hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6F , 6D	Co-expression with phaloidin in cuticular plates of hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlear ganglion	spiral ganglion neuron	Present	Regionally restricted	6G , 6I	Co-expression with Tubb3 in cytoplasm of some but not all spinal ganglion neurons.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	7A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	7F , 7D	Co-expression with calmodulin in cytoplasm of hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	7F , 7D	Co-expression with calmodulin in cytoplasm of hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlear ganglion	spiral ganglion neuron	Present	Regionally restricted	7I , 7G	Expression in a subpopulation of calmodulin-expressing spinal ganglion neuron somata