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Gene Expression Data
Assay Details
Assay
Reference: J:246476 Raum JC, et al., Tshz1 Regulates Pancreatic beta-Cell Maturation. Diabetes. 2015 Aug;64(8):2905-14
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7344316
Gene symbol: Tshz1
Gene name: teashirt zinc finger family member 1
Reporter: GFP
Antibody: anti-GFP
Results
Specimen 2A GFP (upper)`: embryonic day 15.5; Tshz1tm1Garr/Tshz1+ (more )
Note: Triple labeled: green - Tshz1 reporter; red - Ngn3 (Neurog3 assay MGI:7344331); magenta - ChrA (Chga assay MGI:7344339).
Structure Cell Type Level Pattern Image Note
TS23: endocrine pancreas progenitor cell of endocrine pancreas Absent 2A GFP (upper) , 2A (merge upper) No co-expression in Neurog3.
TS23: endocrine pancreas pancreatic endocrine cell Present 2A GFP (upper) , 2A (merge upper) Expression tightly associated with endocrine cell marker expression.
Specimen 2A GFP (lower): embryonic day 15.5; Tshz1tm1Garr/Tshz1+ (more )
Note: Triple labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338); magenta - Gcg (assay MGI:7344342).
Structure Cell Type Level Pattern Image Note
TS23: endocrine pancreas type B pancreatic cell Present 2A GFP (lower) , 2A (merge lower) Expression in insulin-expressing beta cells.
TS23: endocrine pancreas pancreatic A cell Weak 2A (merge lower) , 2A GFP (lower) Lower level of expression in Gcg-expressing alpha cells.
Specimen 2B GFP (upper, lower left): embryonic day 18.5; Tshz1tm1Garr/Tshz1+ (more )
Note: Triple labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338); blue - Gcg (assay MGI:7344342).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas type B pancreatic cell Present 2B GFP (upper) , 2B Ins/GFP , 2B (merge upper) Expression in insulin-expressing beta cells.
TS26: endocrine pancreas pancreatic A cell Weak 2B (merge upper) , 2B GFP (upper) , 2B Ins/GFP Lower level of expression in Gcg-expressing alpha cells.
Specimen 2B GFP lower left-middle): embryonic day 18.5; Tshz1tm1Garr/Tshz1+ (more )
Note: Double labeled: green - Tshz1 reporter; red - Gcg (assay MGI:7344342).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas pancreatic A cell Present 2B Gcg/GFP Expression in Gcg-expressing alpha cells.
Specimen 2B GFP (lower middle-right): embryonic day 18.5; Tshz1tm1Garr/Tshz1+ (more )
Note: Double labeled: green - Tshz1 reporter; red - PP (Ppy assay MGI:7344346).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas pancreatic PP cell Weak 2B PP/GFP Low level of expression in Ppy-expressing cells.
Specimen 2B GFP (lower right): embryonic day 18.5; Tshz1tm1Garr/Tshz1+ (more )
Note: Double labeled: green - Tshz1 reporter; red - SS (Sst assay MGI:7344347).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas pancreatic D cell Weak 2B SS/GFP Low level of expression in delta cells.
Specimen 2C GFP: postnatal week 7; Tshz1tm1Garr/Tshz1+ (more )
Note: Triple labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338); blue - Gcg (assay MGI:7344342).
Structure Cell Type Level Pattern Image Note
TS28: islets of Langerhans type B pancreatic cell Present 2C GFP , 2C (merge) Expression in insulin-expressing beta cells.
TS28: islets of Langerhans pancreatic A cell Weak 2C GFP , 2C (merge) Lower level of expression in Gcg-expressing alpha cells.
Specimen 3A GFP: embryonic day 18.5; Tshz1tm1Garr/Tshz1tm1Garr (more )
Note: Double labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas type B pancreatic cell Present 3A (left) , 3A (right) Expression in insulin-expressing beta cells.
Specimen not shown: embryonic day 18.5; Tshz1tm1Garr/Tshz1tm1Garr (more )
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas pancreatic A cell Present Expression in Gcg-expressing alpha cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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07/29/2025
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