Gene Expression Data
Assay Details
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Assay
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Reference:
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J:246476
Raum JC, et al., Tshz1 Regulates Pancreatic beta-Cell Maturation. Diabetes. 2015 Aug;64(8):2905-14
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7344316
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Gene symbol:
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Tshz1
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Gene name:
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teashirt zinc finger family member 1
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Results
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Specimen
2A GFP (upper)`:
embryonic day 15.5; Tshz1tm1Garr/Tshz1 +
(more  )
Note: Triple labeled: green - Tshz1 reporter; red - Ngn3 (Neurog3 assay MGI:7344331); magenta - ChrA (Chga assay MGI:7344339).
Specimen
2A GFP (upper)`:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tshz1 reporter; red - Ngn3 (Neurog3 assay MGI:7344331); magenta - ChrA (Chga assay MGI:7344339).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: endocrine pancreas |
progenitor cell of endocrine pancreas |
Absent |
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2A GFP (upper)
,
2A (merge upper)
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No co-expression in Neurog3.
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| TS23: endocrine pancreas |
pancreatic endocrine cell |
Present |
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2A GFP (upper)
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2A (merge upper)
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Expression tightly associated with endocrine cell marker expression.
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Specimen
2A GFP (lower):
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338); magenta - Gcg (assay MGI:7344342).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: endocrine pancreas |
type B pancreatic cell |
Present |
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2A GFP (lower)
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2A (merge lower)
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Expression in insulin-expressing beta cells.
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| TS23: endocrine pancreas |
pancreatic A cell |
Weak |
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2A (merge lower)
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2A GFP (lower)
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Lower level of expression in Gcg-expressing alpha cells.
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Specimen
2B GFP (upper, lower left):
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338); blue - Gcg (assay MGI:7344342).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: endocrine pancreas |
type B pancreatic cell |
Present |
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2B GFP (upper)
,
2B Ins/GFP
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2B (merge upper)
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Expression in insulin-expressing beta cells.
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| TS26: endocrine pancreas |
pancreatic A cell |
Weak |
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2B (merge upper)
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2B GFP (upper)
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2B Ins/GFP
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Lower level of expression in Gcg-expressing alpha cells.
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Specimen
2B GFP lower left-middle):
embryonic day 18.5; Tshz1tm1Garr/Tshz1 +
(more )
Note: Double labeled: green - Tshz1 reporter; red - Gcg (assay MGI:7344342).
Specimen
2B GFP lower left-middle):
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: green - Tshz1 reporter; red - Gcg (assay MGI:7344342).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: endocrine pancreas |
pancreatic A cell |
Present |
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2B Gcg/GFP
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Expression in Gcg-expressing alpha cells.
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Specimen
2B GFP (lower middle-right):
embryonic day 18.5; Tshz1tm1Garr/Tshz1 +
(more )
Note: Double labeled: green - Tshz1 reporter; red - PP (Ppy assay MGI:7344346).
Specimen
2B GFP (lower middle-right):
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tshz1 reporter; red - PP (Ppy assay MGI:7344346).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: endocrine pancreas |
pancreatic PP cell |
Weak |
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2B PP/GFP
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Low level of expression in Ppy-expressing cells.
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Specimen
2B GFP (lower right):
embryonic day 18.5; Tshz1tm1Garr/Tshz1 +
(more )
Note: Double labeled: green - Tshz1 reporter; red - SS (Sst assay MGI:7344347).
Specimen
2B GFP (lower right):
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tshz1 reporter; red - SS (Sst assay MGI:7344347).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: endocrine pancreas |
pancreatic D cell |
Weak |
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2B SS/GFP
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Low level of expression in delta cells.
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Specimen
2C GFP:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1+
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338); blue - Gcg (assay MGI:7344342).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
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2C GFP
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2C (merge)
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Expression in insulin-expressing beta cells.
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| TS28: islets of Langerhans |
pancreatic A cell |
Weak |
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2C GFP
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2C (merge)
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Lower level of expression in Gcg-expressing alpha cells.
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Specimen
3A GFP:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1tm1Garr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: green - Tshz1 reporter; red - Ins1 (assay MGI:7344332) and Ins2 (assay MGI:7344338).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: endocrine pancreas |
type B pancreatic cell |
Present |
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3A (left)
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3A (right)
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Expression in insulin-expressing beta cells.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129/Sv * CD-1
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Mutant Allele(s):
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Tshz1tm1Garr/Tshz1tm1Garr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: endocrine pancreas |
pancreatic A cell |
Present |
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Expression in Gcg-expressing alpha cells.
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Analysis Tools