Antibody:	anti-DOPA decarboxylase antibody (ab3905)
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Structure	Level	Pattern	Image	Note
TS23: interventricular septum	Present		1B , 1A
TS23: ventricle cardiac muscle	Present		1B , 1A
TS23: endocardial lining	Absent		1A , 1B
TS23: heart atrium	Absent		1A , 1B
TS23: visceral pericardium	Absent		1A , 1B
TS23: trabecular myocardium	Present	Regionally restricted	1A , 1B	Expression was only found in the trabeculae where it meets the compact myocardial layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: ventricle cardiac muscle		Present	Scattered	1D , 1E	Expression was scattered throughout the compact myocardium of both ventricles.
TS23: interventricular septum		Present		1E , 1D
TS23: endocardial lining		Absent		1D , 1E
TS23: heart atrium		Absent		1D , 1E
TS23: visceral pericardium		Absent		1D , 1E
TS23: trabecular myocardium		Present	Regionally restricted	1D , 1E	Expression was only found in the trabeculae where it meets the compact myocardial layer.
TS23: right ventricle cardiac muscle	cardiac muscle cell	Present	Scattered	1D , 1E	Expression was detected in the myocytes of the right ventricular apex.
TS23: outflow tract	cardiac muscle cell	Present		1D , 1E
TS23: interventricular septum	cardiac muscle cell	Present	Regionally restricted	1D , 1E	Expression was detected in myocytes of the right ventricular portion of the interventricular septum.

Structure	Level	Pattern	Image	Note
TS23: interventricular septum	Present		1H , 1G
TS23: ventricle cardiac muscle	Present		1H , 1G
TS23: trabecular myocardium	Present	Regionally restricted	1H , 1G	Expression was only found in the trabeculae where it meets the compact myocardial layer.
TS23: endocardial lining	Absent		1H , 1G
TS23: visceral pericardium	Absent		1H , 1G

Structure	Cell Type	Level	Pattern	Image	Note
TS26: right ventricle cardiac muscle	cardiac muscle cell	Present	Regionally restricted	1J	Expression was detected in myocytes of the right ventricular apex.
TS26: interventricular septum	cardiac muscle cell	Present	Regionally restricted	1J	Expression was detected in myocytes of the right ventricular portion of the interventricular septum.
TS26: outflow tract	cardiac muscle cell	Present		1J

Structure	Cell Type	Level	Pattern	Image	Note
TS26: right ventricle cardiac muscle	cardiac muscle cell	Present	Regionally restricted	1K	Expression was detected in the myocytes of the right ventricular apex.
TS26: outflow tract	cardiac muscle cell	Present		1K
TS26: interventricular septum	cardiac muscle cell	Present	Regionally restricted	1K	Expression was detected in myocytes of the right ventricular portion of the interventricular septum.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: right ventricle cardiac muscle	cardiac muscle cell	Present	Regionally restricted	1L	Expression was detected in the myocytes of the right ventricular apex.
TS26: outflow tract	cardiac muscle cell	Present		1L
TS26: interventricular septum	cardiac muscle cell	Present	Regionally restricted	1L	Expression was detected in myocytes of the right ventricular portion of the interventricular septum.