Antibody:	Anti-LGR5 (HPA012530)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS18: midbrain ventricular layer	Present	Regionally restricted	2A left , 2A right	Expressed in cells lining the ventricle in the ventral midbrain.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: midbrain	radial glial cell	Strong		2B lower right , 2B upper left , 2B lower left , 2B upper right	Less positive cells bodies and a strong staining radial processes co-stained with Nes.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present		2C upper right , 2C upper left	Decreased expression. Sporadic positive cell bodies in the ventricular zone, positive fibers were more restricted to the ventral midbrain domain.
TS20: midbrain ventricular layer	Present		2C upper right , 2C upper left	Decreased expression. Sporadic positive cell bodies in the ventricular zone, positive fibers were more restricted to the ventral midbrain domain.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present		2C lower right , 2C lower left	Sporadic positive cell bodies (coexpressed with Sox2) in the ventricular zone, positive fibers were more restricted to the ventral midbrain domain.
TS20: midbrain ventricular layer	Present		2C lower right , 2C lower left	Sporadic positive cell bodies (coexpressed with Sox2) in the ventricular zone, positive fibers were more restricted to the ventral midbrain domain.