Gene Expression Data
Assay Details
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Assay
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Reference:
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J:312419
Haddad-Tovolli R, et al., Mouse thalamic differentiation: gli-dependent pattern and gli-independent prepattern. Front Neurosci. 2012;6:27
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7284823
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Results
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Specimen
4D:
embryonic day 18.5
(more  )
Note: Double labeled: green - Nefm; red - Hes1 (assay MGI:7284824).
Specimen
4D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nefm; red - Hes1 (assay MGI:7284824).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: thalamus |
neuron |
Present |
Regionally restricted |
4D
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Expressed in thalamocortical axons streaming out of the ventral posterior group.
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Specimen
4E:
embryonic day 18.5; Gli3Xt/ Gli3Xt
(more )
Note: Double labeled: green - Nefm; red - Hes1 (assay MGI:7284824).
Specimen
4E:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nefm; red - Hes1 (assay MGI:7284824).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: thalamus |
neuron |
Present |
Regionally restricted |
4E
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Expressed in thalamocortical axons streaming out of the ventral posterior group.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nefm; red - Hes1 (assay MGI:7284824).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: thalamus |
neuron |
Present |
Regionally restricted |
4F
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Expressed in thalamocortical axons streaming out of the ventral posterior group with a reduced number.
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Specimen
4G:
embryonic day 18.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
neuron |
Present |
Regionally restricted |
4G
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Expressed in the thalamocortical axons forming a well-developed layer in the cortex.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
neuron |
Present |
Regionally restricted |
4H
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Expressed in very large numbers of thalamocortical axons, which had not been able to proceed further into the cortex region.
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Specimen
4I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
neuron |
Present |
Regionally restricted |
4I
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Expressed in very sparse thalamocortical axons, resulting in a thin labeled axonal layer in the cortex.
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Specimen
6B/C:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * CBA/H
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Mutant Allele(s):
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Foxb1tm1(cre)Gabo/Foxb1+
Shhtm2Amc/Shhtm2Amc
Gli3Xt/Gli3Xt
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Double labeled: green - Nefm; red - Hes1 (assay MGI:7284824).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
neuron |
Present |
Regionally restricted |
6B
,
6C
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Expressed in thalamocortical axons, not very numerous, extending from the thalamic primordium in the direction of the telencephalon. The axons seemed unable to enter the telencephalic primordium.
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