Antibody:	Anti-EXOSC10/RRP6 antibody (ab50558)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Diffuse	2E Oocyte	Diffuse cytoplasmic expression.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage conceptus	Present	Diffuse	2E Zygote	Diffuse signal in the cytoplasm and the pronuclei.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage conceptus	Present		2E 2-cell	Accumulated in the nucleus.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		2E 4-cell	Accumulated in the nucleus.

Structure	Level	Pattern	Image	Note
TS4: morula-stage conceptus	Present		2E Morula	Accumulated in the nucleus.

Structure	Level	Pattern	Image	Note
TS4: blastocyst-stage conceptus	Present		2E early Blast.	Accumulated in the nucleus.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage conceptus	Weak	Diffuse	3A 2-cell	Diffuse cytoplasmic expression.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		3A 3-cell	Expressed in newly formed nuclei and diffuse cytoplasmic expression.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		3B EXOSC10 , 3B merge	Accumulated in the nucleus and foci. Not co-localized with Srsf2.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		3C EXOSC10 , 3C merge	Accumulated in the nucleus and foci. Not co-localized with Sfpq.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A1a EXOSC10 , 4A1a merge	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A1b merge , 4A1b EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A1c merge , 4A1c EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A1d merge , 4A1d EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A1e EXOSC10 , 4A1e merge	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A1f merge , 4A1f EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A4g EXOSC10 , 4A4g merge	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A4h EXOSC10 , 4A4h merge	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A4i EXOSC10 , 4A4i merge	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A4j EXOSC10 , 4A4j merge	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A2k merge , 4A2k EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage conceptus	Present		4A2l merge , 4A2l EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage conceptus	Present		4B merge , 4B EXOSC10	Accumulated discontinuously at the nucleolus precursor body periphery or completely surrounded the nucleolus precursor bodies, where it colocalized with the nucleolar marker Npm1.