Antibody:	Anti-Hof
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	4A	Expression was confined to the archicortical neuroepithelium lining the lateral ventricle.
TS24: hippocampus	Present	Regionally restricted	4A	Expression was detected in the hippocampal plate.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	4B	Expression was confined to the archicortical neuroepithelium lining the lateral ventricle.
TS26: hippocampus	Present	Regionally restricted	4B	Expression was detected in the hippocampal intermediate zone and the hippocampal plate.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present		4B upper inset
TS26: neocortex	Absent		4B upper inset

Structure	Level	Pattern	Image	Note
TS26: dentate notch	Present	Regionally restricted	4B lower inset	Zbtb20-expressing progenitors leave the dentate notch to migrate to the dentate gyrus.
TS26: dentate gyrus	Present		4B lower inset

Structure	Cell Type	Level	Pattern	Image	Note
TS27: hippocampus		Present	Regionally restricted	4C	Expression was detected in the migrating cells of the hippocampal intermediate zone.
TS27: hippocampal formation		Present	Regionally restricted	4C	Expression was detected along the dentate gyrus migratory path.
TS27: hippocampus CA1	hippocampal pyramidal neuron	Present		4C
TS27: hippocampus CA3	hippocampal pyramidal neuron	Present		4C
TS27: dentate gyrus	dentate gyrus granule cell	Present	Regionally restricted	4C	Expression was detected in granule cells settled in the external blade of the dentate gyrus.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	hippocampal pyramidal neuron	Strong		4D
TS28: dentate gyrus	dentate gyrus granule cell	Strong		4D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus granule cell layer	neuron	Strong	Graded	4D inset	A gradient of expression was noted. Highest levels occurred in the in the newest born cells, and there was a marked decline of expression in mature neurons expressing the marker Rbfox3.
TS28: hippocampus pyramidal cell layer	neuron	Strong	Graded	4D inset	A gradient of expression was noted. Highest levels occurred in the in the newest born cells, and there was a marked decline of expression in mature neurons expressing the marker Rbfox3.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	hippocampal pyramidal neuron	Present		4E	A decline in expression was noted in the pyramidal neurons after the end of the second postnatal week.
TS28: dentate gyrus hilus	dentate gyrus granule cell	Strong		4E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus	dentate gyrus granule cell	Strong	Regionally restricted	4E inset	High expression was noted in less mature granule neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	neuron	Moderate		4F
TS28: dentate gyrus	dentate gyrus granule cell	Moderate		4F

Structure	Level	Pattern	Image	Note
TS26: hippocampus CA1	Present		4K	Expression was detected in migrating and settled CA1 cells.

Structure	Level	Pattern	Image	Note
TS26: indusium griseum	Present		4M

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present		4N , 4P	Staining was nuclear and did not co-stain with BrdU.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	cerebellar granule cell	Present	Regionally restricted	5A	Expression was in the nuclei of immature migrating granule neurons in the molecular layer.
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present	Regionally restricted	5A	Expression was in a transient layer in the upper aspect of the internal granule cell layer, as well as in scattered cells in deeper layers of the internal granule cells layer. Cells in the internal granule cell layer do not co-express with Rbfox3.
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Diffuse	5A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	cerebellar granule cell	Present	Regionally restricted	5B	Expression was in the nuclei of immature migrating granule neurons in the molecular layer.
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present	Regionally restricted	5B	Expression was in a transient layer in the upper aspect of the internal granule cell layer, as well as in scattered cells in deeper layers of the internal granule cells layer. Cells in the internal granule cell layer do not co-express with Rbfox3.
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Diffuse	5B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	cerebellar granule cell	Present	Regionally restricted	5C	Expression was in the nuclei of immature migrating granule neurons in the molecular layer.
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present	Regionally restricted	5C	Expression was in a transient layer in the upper aspect of the internal granule cell layer, as well as in scattered cells in deeper layers of the internal granule cells layer.
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Diffuse	5C

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		5D
TS28: cerebellum molecular layer	Present	Regionally restricted	5D	Expression in the molecular layer is restricted to a narrow band of cells lining the pial surface, as well as in scattered cells along the migratory path to the internal granule cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present		5E	Only a minor portion of the cells co-express with Rbfox3.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Weak	Scattered	5F	Expression in the granule cell layer has declined, and staining is confined to a few scattered cells.
TS28: cerebellum molecular layer	Weak	Scattered	5F

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Moderate	Non-Uniform	6A	Weak to moderate levels of immunostaining.
TS27: rostral migratory stream	Moderate	Non-Uniform	6A	Weak to moderate levels of immunostaining.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		6B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present	Regionally restricted	6C	Expression was detected in the anterior subventricular zone.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6D	Expression was detected in numerous cells migrating along the vertical limb of the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6E	Expression was detected in numerous cells migrating along the vertical limb of the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Scattered	6F	Expression was not confined to a single layer but was scattered throughout most of the regions of the bulb.
TS28: main olfactory bulb glomerular layer	Present	Regionally restricted	6F	Expressing cells were found mainly at the periphery of individual glomeruli with a few positive cells at the center.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6I , 6H	Expression was detected in the horizontal limb of the rostral migratory stream.
TS28: main olfactory bulb granule cell layer	Present	Scattered	6H , 6I	Zbtb20-expressing cells did not co-express Rbfox3.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6J	Expression was confined to a subpopulation of cells migrating in the horizontal limb of the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present	Regionally restricted	6K	Zbtb20-expressing cells did not co-express Rbfox3.
TS28: rostral migratory stream	Present	Regionally restricted	6K	Zbtb20-expressing cells did not co-express Rbfox3.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	astrocyte	Present	Regionally restricted	6L	The majority of Zbtb20-expressing cells express the astroglial marker Gfap.
TS28: rostral migratory stream	astrocyte	Present	Regionally restricted	6L	The majority of Zbtb20-expressing cells express the astroglial marker Gfap.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum white matter	glial cell	Present	Regionally restricted	7B	Only a subpopulation of Zbtb20-expressing glial cells co-express Gfap.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum white matter	oligodendrocyte	Present	Regionally restricted	7C	Oligodendroglia in the cerebellar white matter co-expressed Cnp and Zbtb20.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: fornix	oligodendrocyte	Present		7F , 7D

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present	Regionally restricted	7J , 7L	Expression was detected in the gliogenic subventricular zone of the fimbria. The Zbtb20-positive cells lacked staining for BrdU.