Gene Expression Data
Assay Details
Assay
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Reference:
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J:137801
Nakayama A, et al., Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo. J Comp Neurol. 2008 Jul 10;509(2):211-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6885883
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Gene symbol:
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Prox1
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Gene name:
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prospero homeobox 1
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Probe:
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Prox1 probe7
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Probe preparation:
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Antisense RNA
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Visualized with:
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Fluorescence
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Results
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Specimen
2A/C:
embryonic day 12.5
(more )
Note: Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
Specimen
2A/C:
(close )
Genetic Background:
|
C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
|
Specimen
2E/G:
embryonic day 14.5
(more )
Note: Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
Specimen
2E/G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
|
Specimen
2I/K:
postnatal adult
(more )
Note: Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
Specimen
2I/K:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: fungiform papillae |
Strong |
Regionally restricted |
2K
,
2I
|
Strong expression in basal cells; weak expression spread into entire taste bud.
|
Specimen
6A/C:
embryonic day 14.5
(more )
Note: Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
Specimen
6A/C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: soft palate |
Present |
Regionally restricted |
6C
,
6A
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Expression in same small collections of cells, slighly elongated in direction perpendicular to epithelial surface, as Shh.
|
Specimen
6E/G:
embryonic day 16.5
(more )
Note: Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
Specimen
6E/G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: soft palate |
Present |
Regionally restricted |
6E
,
6G
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Expression in elongated cells. Strong expression restricted to a subset of Shh-expressing cells.
|
Specimen
6I/K:
postnatal adult
(more )
Note: Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
Specimen
6I/K:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: pink - Prox1; green - Shh (assay MGI:6885880).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: soft palate |
Strong |
Regionally restricted |
6K
,
6I
|
Strong expression in basal cells, overlapping with Shh expression.
|
|