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Gene Expression Data
Assay Details
Assay
Reference: J:314664 Harada Y, et al., Cell cycle arrest determines adult neural stem cell ontogeny by an embryonic Notch-nonoscillatory Hey1 module. Nat Commun. 2021 Nov 12;12(1):6562
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6860526
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: anti-Gfap (ab4674)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 5e Hey1+/+: postnatal day 21 (more )
Note: Triple labeled: green - Gfap; white - S100b (assay MGI:6860527); red - Sox2 (assay MGI:6860525).
Structure Level Pattern Image Note
TS28: brain subventricular zone Present Regionally restricted 5e Hey1+/+ (upper) , 5e Hey1+/+ (lower) Expression in neural stem cells (NSCs).
Specimen 5e Hey1-/-: postnatal day 21; Hey1tm1Kkb/Hey1tm1Kkb (more )
Note: Triple labeled: green - Gfap; white - S100b (assay MGI:6860527); red - Sox2 (assay MGI:6860525).
Structure Level Pattern Image Note
TS28: brain subventricular zone Weak Regionally restricted 5e Hey1-/- (lower) , 5e Hey1-/- (upper) Expression significantly reduced in subventricular zone (SVZ) of knockout compared to control.
Specimen 5f Hey1+/+: postnatal day 21 (more )
Note: Triple labeled: green - Gfap; red - Egfr (assay MGI:6860528); white - S100b (assay MGI:6860527).
Structure Level Pattern Image Note
TS28: brain subventricular zone Present Regionally restricted 5f Hey1+/+ (upper) , 5f Hey1+/+ (lower) No expression in transit amplifying cells.
Specimen 5f Hey1-/-: postnatal day 21; Hey1tm1Kkb/Hey1tm1Kkb (more )
Note: Triple labeled: green - Gfap; red - Egfr (assay MGI:6860528); white - S100b (assay MGI:6860527).
Structure Level Pattern Image Note
TS28: brain subventricular zone Weak Regionally restricted 5f Hey1-/- (lower) , 5f Hey1-/- (upper)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory