Gene Expression Data
Assay Details
Assay
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Reference:
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J:181053
Beyer LA, et al., Age-related changes in expression of CTL2/SLC44A2 and its isoforms in the mouse inner ear. Hear Res. 2011 Dec;282(1-2):63-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6830946
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Gene symbol:
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Slc44a2
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Gene name:
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solute carrier family 44, member 2
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Antibody:
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anti CTL2-NT
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A:
embryonic day 17.0
(more )
Note: Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
Specimen
3A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: utricle |
Present |
Regionally restricted |
3A
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Expression is in supporting cells and hair cells.
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Specimen
3B:
postnatal day 1
(more )
Note: Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
Specimen
3B:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal day 1
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
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2% Paraformaldehyde
|
Note:
|
Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: utricle |
Present |
Regionally restricted |
3B
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Expression is in supporting cells and a sub-population of hair cells.
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Specimen
3C:
postnatal day 7
(more )
Note: Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
Specimen
3C:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: utricle |
Present |
Regionally restricted |
3C
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Expression is in supporting cells and is barely detetcable in a sub-population of hair cells.
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Specimen
4A:
embryonic day 17.0
(more )
Specimen
4A:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.0
|
Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: organ of Corti |
Present |
Regionally restricted |
4A
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Expression is in the pillar cells, the inner phalangeal cells, first row Deiters cells, and in many hair cells.
|
Specimen
4B:
postnatal day 1
(more )
Note: Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
Specimen
4B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 1
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Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
2% Paraformaldehyde
|
Note:
|
Double labeled: green - Slc44a2; red - Myo7a (assay MGI:6830937).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Present |
Regionally restricted |
4B
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Expression is stronger in the second and third row of outer hair cells than the first row. Expression is mainly in supporting cells including the pillars and inner phalangeal cells.
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Specimen
4C:
postnatal day 7
(more )
Note: Counterstained with phalloidin (red).
Specimen
4C:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
Counterstained with phalloidin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
4C
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Expression is in all supporting cells, with strongest expression in the inner and outer pillars, hair cells are negative.
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Specimen
5:
postnatal day 20
(more )
Note: Counterstained with phalloidin (red).
Specimen
5:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal day 20
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
2% Paraformaldehyde
|
Note:
|
Counterstained with phalloidin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
5
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Expression is in supporting cells and along the membrane of the Deiters cells and pillar cells.
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Specimen
not shown E18:
embryonic day 18.0
(more )
Specimen
not shown E18:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 18.0
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: utricle |
Present |
Regionally restricted |
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Expression is in developing supporting cells and sensory cells.
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TS26: cochlea |
Present |
Regionally restricted |
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Expression is in some hair cells.
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