Gene Expression Data
Assay Details
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Assay
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Reference:
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J:308106
Jang S, et al., Clmp Regulates AMPA and Kainate Receptor Responses in the Neonatal Hippocampal CA3 and Kainate Seizure Susceptibility in Mice. Front Synaptic Neurosci. 2020;12:567075
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6829696
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Gene symbol:
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Gad1
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Gene name:
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glutamate decarboxylase 1
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Probe:
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Mm-Gad1 (400951)
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Probe preparation:
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Antisense
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Visualized with:
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Fluorescence
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Assay notes:
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RNAscope assay using the C3 probe conjugated to Atto 647.
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Results
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Specimen
1C (lower):
postnatal day 7
(more  )
Note: Triple labeled: white - Gad1; red - Clmp (assay MGI:6829691); green - Gad2 (assay MGI:6829695). Also stained with DAPI (blue).
Specimen
1C (lower):
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: white - Gad1; red - Clmp (assay MGI:6829691); green - Gad2 (assay MGI:6829695). Also stained with DAPI (blue).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
Regionally restricted |
1C Whole brain Merge (lower)
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1C Whole brain (lower)
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1C Whole brain Gad1
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Expression is seen in GABAergic neurons.
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| TS28: cerebral cortex |
Present |
Regionally restricted |
1C CC deep layer Merge (lower)
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1C CC deep layer Gad1
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1C CC upper layer Gad1
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1C Whole brain (lower)
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1C CC upper layer Merge (lower)
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Expression is seen in GABAergic neurons.
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| TS28: hippocampus CA3 |
Present |
Regionally restricted |
1C CA3 Gad1
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1C CA3 Merge (lower)
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1C Whole brain (lower)
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Expression is seen in GABAergic neurons.
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| TS28: dentate gyrus |
Present |
Regionally restricted |
1C DG Gad1
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1C DG Merge (lower)
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1C Whole brain (lower)
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Expression is seen in GABAergic neurons.
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| TS28: hippocampus CA1 |
Present |
Regionally restricted |
1C Whole brain (lower)
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1C CA1 Gad1
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1C CA1 Merge (lower)
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Expression is seen in GABAergic neurons.
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Specimen
S2B:
postnatal day 56
(more )
Note: Triple labeled: white - Gad1; red - Clmp (assay MGI:6829691); green - Gad2 (assay MGI:6829695). Also stained with DAPI (blue).
Specimen
S2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 56
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Triple labeled: white - Gad1; red - Clmp (assay MGI:6829691); green - Gad2 (assay MGI:6829695). Also stained with DAPI (blue).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
Regionally restricted |
S2B Whole brain
,
S2B Whole brain Merge
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S2B Whole brain Gad1
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Expression is seen in GABAergic neurons.
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| TS28: cerebral cortex |
Present |
Regionally restricted |
S2B CC deep layer Merge
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S2B CC deep layer Gad1
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S2B CC upper layer Gad1
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S2B Whole brain
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S2B CC upper layer Merge
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Expression is seen in GABAergic neurons.
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| TS28: hippocampus CA3 |
Present |
Regionally restricted |
S2B Whole brain
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S2B CA3 Gad1
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S2B CA3 Merge
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Expression is seen in GABAergic neurons.
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| TS28: dentate gyrus |
Present |
Regionally restricted |
S2B Whole brain
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S2B DG Gad1
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S2B DG Merge
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Expression is seen in GABAergic neurons.
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| TS28: hippocampus CA1 |
Present |
Regionally restricted |
S2B CA1 Gad1
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S2B CA1 Merge
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S2B Whole brain
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Expression is seen in GABAergic neurons.
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Analysis Tools