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Gene Expression Data
Assay Details
Assay
Reference: J:307862 Wilkerson BA, et al., Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris. Elife. 2021 May 18;10:e60108
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6828882
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: Anti-Sox2 (Y-17; sc-17320)
Detection system: Secondary antibody
Results
Specimen 2i: postnatal day 0 (more )
Note: Double labeled: green - Sox2; magenta - Id1 (assay MGI:6828881). Also stained with phalloidin (blue).
Structure Level Pattern Image Note
TS27: crista ampullaris Present Regionally restricted 2i Expression is seen in support cells.
Specimen 2i': postnatal day 0 (more )
Note: Double labeled: green - Sox2; magenta - Id1 (assay MGI:6828881). Also stained with phalloidin (blue).
Structure Level Pattern Image Note
TS27: crista ampullaris Present Regionally restricted 2i' Expression is seen in support cells.
Specimen 2j: postnatal day 0 (more )
Note: Triple labeled: magenta - Sox2; green - Atoh1 (assay MGI:6828883); blue - Myo7a (assay MGI:6828884).
Structure Level Pattern Image Note
TS27: crista ampullaris Present Regionally restricted 2j'' , 2j''' Expression is seen in support cells.
Specimen 2 S1: postnatal day 3 (more )
Note: Triple labeled: blue - Sox2; green - Ocm (assay MGI:6828888); magenta - Nefm (assay MGI:6828889).
Structure Level Pattern Image Note
TS27: crista ampullaris Present Regionally restricted 2 S1_merge , 2 S1_blue Expression is seen in vestibular type II hair cell subtypes.
Specimen 2 S6c: postnatal day 3 (more )
Note: Double labeled: magenta - Sox2; yellow - Hspa1a (assay MGI:6828989). Also stained with phalloidin (blue).
Structure Level Pattern Image Note
TS27: crista ampullaris Present Regionally restricted 2 S6c merge , 2 S6c magenta Expression is seen in the support cell layer.
Specimen 2 S6d: postnatal day 7 (more )
Note: Double labeled: magenta - Sox2; yellow - Hspa1a (assay MGI:6828989). Also stained with phalloidin (blue).
Structure Level Pattern Image Note
TS28: crista ampullaris Present Regionally restricted 2 S6d magenta , 2 S6d merge Expression is seen in the support cell layer.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory