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Gene Expression Data
Assay Details
Assay
Reference: J:307957 Xie X, et al., alpha-TubK40me3 is required for neuronal polarization and migration by promoting microtubule formation. Nat Commun. 2021 Jul 5;12(1):4113
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6828776
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: TuJ1 (ab107216)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1c - E14: embryonic day 14.0 (more )
Note: Also stained for methylated alpha-tubulin (red) and DAPI (blue).
Structure Level Pattern Image Note
TS22: cortical plate Present 1c E14 CP Tuj1 , 1c E14 Tuj1 (low mag)
TS22: cerebral cortex intermediate zone Present 1c E14 Tuj1 (low mag)

Specimen 1c - E16: embryonic day 16.0 (more )
Note: Also stained for methylated alpha-tubulin (red) and DAPI (blue).
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Present 1c E16 Tuj1
TS24: cortical plate Present 1c E16 Tuj1

Specimen 1c - P0: postnatal day 0 (more )
Note: Also stained for methylated alpha-tubulin (red) and DAPI (blue).
Structure Level Pattern Image Note
TS27: cerebral cortex Present 1c P0 Tuj1

Specimen 2b - E14: embryonic day 14.0 (more )
Note: Double labeled: green - Tubb3; red - Setd2 (assay MGI:6828778). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present 2b E14 Merge , 2b E14 Tuj1
TS22: cortical plate Present 2b E14 Merge , 2b E14 Tuj1

Specimen 2b - E16: embryonic day 16.0 (more )
Note: Double labeled: green - Tubb3; red - Setd2 (assay MGI:6828778). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Present 2b E16 Tuj1 , 2b E16 Merge
TS24: cortical plate Present 2b E16 Tuj1 , 2b E16 Merge


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory