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Gene Expression Data
Assay Details
Assay
Reference: J:306255 Ivanovitch K, et al., Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. PLoS Biol. 2021 May;19(5):e3001200
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6782401
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachyury (AF2085)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 4A: embryonic day 6.85 (more )
Note: Mid-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present 4A merge , 4A T
Specimen 4B: embryonic day 6.85 (more )
Note: Mid-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present 4Biii , 4B
TS10: definitive endoderm Present 4Bi , 4B , 4Bii
Specimen 4C: embryonic day 7.0 (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present 4C T , 4C merge
TS10: node Present 4C merge , 4C T Expression is seen at the distal tip of the embryo where the node forms.
Specimen 4D: embryonic day 7.0 (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: definitive endoderm Present 4D , 4Di
TS10: node Present 4Dii , 4D
TS10: primitive streak Present 4Diii , 4D
Specimen 4E: embryonic day 7.25 (more )
Note: Early bud stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present 4E T , 4E merge
TS10: node Present 4E T , 4E merge
Specimen 4F: embryonic day 7.25 (more )
Note: Early bud stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: node Present 4F , 4Fii
TS10: primitive streak Present 4F , 4Fiii
Specimen S6A: embryonic day 6.85 (more )
Note: Mid-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present S6A
TS10: definitive endoderm Present S6A , S6Aii , S6Ai
Specimen S6B: embryonic day 6.85 (more )
Note: Mid-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present S6B'' , S6B , S6Bi
Specimen S6C: embryonic day 7.0 (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present S6Ci , S6C'' , S6C
Specimen S6D: embryonic day 7.25 (more )
Note: Early bud stage embryo. Double labeled: red - T; green - Foxa2 (assay MGI:6782403).
Structure Level Pattern Image Note
TS10: primitive streak Present S6D'' , S6D , S6Di
TS10: node Present S6D , S6D''
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory