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Gene Expression Data
Assay Details
Assay
Reference: J:272572 May AJ, et al., Diverse progenitor cells preserve salivary gland ductal architecture after radiation-induced damage. Development. 2018 Nov 5;145(21):dev166363
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6757231
Gene symbol: Krt14
Gene name: keratin 14
Antibody: Anti-Keratin 14 (AF64, PRB-155P)
Detection system: Secondary antibody coupled to DyLight 488
Results
Specimen 1A: postnatal week 6 (more )
Note: Triple labeled: green - Krt14; blue - Cdh1 (assay MGI:6758242); red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS28: submandibular gland Present Regionally restricted 1A middle , 1A left Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells.
Specimen 1C: embryonic day 15.0 (more )
Note: Double labeled: green - Krt14; red - Kit (assay MGI:6758237).
Structure Level Pattern Image Note
TS23: submandibular gland Present 1C upper left , 1C upper middle Few Krt14+ cells expressed Kit.
Specimen 2A P2 upper: postnatal day 2 (more )
Note: Double labeled: green - Krt14; red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS27: submandibular duct Present 2A P2 upper Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells (46%).
Specimen 2A P2 lower: postnatal day 2 (more )
Note: Triple labeled: green - Krt14; red - EdU; blue - Acta2 (assay MGI:6758283).
Structure Level Pattern Image Note
TS27: submandibular duct Present 2A P2 lower Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells (46%, also proliferative).
Specimen 2A P7 upper: postnatal day 7 (more )
Note: Double labeled: green - Krt14; red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS28: submandibular duct Present 2A P7 upper Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells. Apical expression in initiating granulated duct cells.
Specimen 2A P7 lower: postnatal day 7 (more )
Note: Triple labeled: green - Krt14; red - EdU; blue - Acta2 (assay MGI:6758283).
Structure Level Pattern Image Note
TS28: submandibular duct Present 2A P7 lower Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells (also proliferative). Apical expression in initiating granulated duct cells.
Specimen 2A P14 upper: postnatal day 14 (more )
Note: Double labeled: green - Krt14; red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS28: submandibular duct Present 2A P14 upper Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells (73%).
Specimen 2A P14 lower: postnatal day 14 (more )
Note: Triple labeled: green - Krt14; red - EdU; blue - Acta2 (assay MGI:6758283).
Structure Level Pattern Image Note
TS28: submandibular duct Present 2A P14 lower Expressed in Acta2+ myoepithelial cells, and Acta2- basal duct cells (73%, also proliferative).
Specimen 2A P30 upper: postnatal day 30 (more )
Note: Double labeled: green - Krt14; red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS28: submandibular duct Present Regionally restricted 2A P30 upper No longer expressed in most granulated duct cells. Expressed in ductal progenitors at the acinar-intercalated duct and intercalated duct-granulated duct junctions (39%).
Specimen 2A P30 lower: postnatal day 30 (more )
Note: Triple labeled: green - Krt14; red - EdU; blue - Acta2 (assay MGI:6758283).
Structure Level Pattern Image Note
TS28: submandibular duct Present Regionally restricted 2A P30 lower No longer expressed in most granulated duct cells (also proliferative). Expressed in ductal progenitors at the acinar-intercalated duct and intercalated duct-granulated duct junctions (39%).
Specimen 6A control: postnatal adult (more )
Note: Triple labeled: green - Krt14; purple - EdU; red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS28: submandibular duct Present 6A upper merge , 6A upper Krt14 Expressed in myoepithelial cells and basal duct cells.
Specimen 6G control: postnatal adult (more )
Note: Triple labeled: green - Krt14; purple - EdU; red - Acta2 (assay MGI:6757239).
Structure Level Pattern Image Note
TS28: submandibular duct Present 6G upper merge , 6G upper Krt14 Expressed in myoepithelial cells and basal duct cells.
Specimen S1B: postnatal adult (more )
Note: Triple labeled: green - Krt14; red - Muc19 (assay MGI:6758241); blue - Acta2 (assay MGI:6758283).
Structure Level Pattern Image Note
TS28: submandibular duct Present Regionally restricted S1B right , S1B left Expressed in cells located distal to the granulated duct (Acta2-Muc19-).
Specimen S1C: postnatal day 30 (more )
Note: Double labeled: green - Krt14; red - Kit (assay MGI:6758237).
Structure Level Pattern Image Note
TS28: submandibular duct Present S1C middle , S1C left Expressed in ductal progenitors and myoepithelial cells. No longer co-expressed with Kit.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory