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Gene Expression Data
Assay Details
Assay
Reference: J:288632 McGinty JW, et al., Tuft-Cell-Derived Leukotrienes Drive Rapid Anti-helminth Immunity in the Small Intestine but Are Dispensable for Anti-protist Immunity. Immunity. 2020 Mar 17;52(3):528-541.e7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6757199
Gene symbol: Dclk1
Gene name: doublecortin-like kinase 1
Antibody: anti-DCLK1 (ab31704)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4A WT: postnatal week 6-12 (more )
Note: Uninfected animal.
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells 4A upper left Expression is seen in tuft cells in the proximal small intestine.
Specimen 4A -/-: postnatal week 6-12; Alox5tm1Fun/Alox5tm1Fun (more )
Note: Uninfected animal.
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells 4A lower left Expression is seen in tuft cells in the proximal small intestine; their number is the same in the mutant as in wild type.
Specimen 5C: postnatal week 6-12 (more )
Note: Uninfected animal. Double labeled: red - Dclk1; gray - Alox5 (assay MGI:6757200).
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells 5C merge , 5C red Expression is seen in tuft cells in the proximal small intestine.
Specimen 7H - proximal: postnatal week 6-12 (more )
Note: Uninfected animal. Double labeled: red - Dclk1; gray - Alox5 (assay MGI:6757200).
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells 7H upper Expression is seen in tuft cells in the proximal small intestine.
Specimen 7H - distal: postnatal week 6-12 (more )
Note: Uninfected animal. Double labeled: red - Dclk1; gray - Alox5 (assay MGI:6757200).
Structure Level Pattern Image Note
TS28: small intestine epithelium Present Single cells 7H lower Expression is seen in tuft cells in the distal small intestine.
Specimen S5B: postnatal week 6-12 (more )
Note: Uninfected animal. Double labeled: red - Dclk1; gray - Alox5 (assay MGI:6757200).
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells S5B left Expression is seen in tuft cells in the proximal small intestine.
Specimen S6D: postnatal week 6-12; Pou2f3tm1.1(cre/ERT2)Imt/Pou2f3+ (more )
Note: Uninfected animal. Double labeled: red - Dclk1; green - Pou2f3 reporter (assay MGI:6755198).
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells S6D merge , S6D red Expression is seen in tuft cells in the proximal small intestine.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory