Gene Expression Data
Assay Details
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Assay
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Reference:
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J:243896
Chevalier M, et al., The embryonic development of hindbrain respiratory networks is unaffected by mutation of the planar polarity protein Scribble. Neuroscience. 2017 Aug 15;357:160-171
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6755557
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Gene symbol:
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Isl2
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Gene name:
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insulin related protein 2 (islet 2)
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Antibody:
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anti-islet 1,2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1C +/+:
embryonic day 14.5
(more  )
Specimen
1C +/+:
(close  )
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Genetic Background:
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involves: C57BL/6J * NMRI
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1C crc/+:
embryonic day 14.5; ScribCrc/Scrib +
(more )
Specimen
1C crc/+:
(close )
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Genetic Background:
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involves: C57BL/6J * NMRI
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Mutant Allele(s):
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ScribCrc/Scrib+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindbrain |
Present |
Regionally restricted |
1C crc/+
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Expression is in several motoneuronal populations.
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| TS22: facial nucleus |
Present |
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1C crc/+
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Specimen
1C crc/crc:
(close )
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Genetic Background:
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involves: C57BL/6J * NMRI
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Mutant Allele(s):
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ScribCrc/ScribCrc
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindbrain |
Present |
Regionally restricted |
1C crc/crc
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Expression is in several motoneuronal populations.
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| TS22: facial nucleus |
Present |
Regionally restricted |
1C crc/crc
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This nucleus is located in an abnormal medial position in this mutant compared to wild-type.
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Specimen
3B:
embryonic day 16.5
(more )
Note: Double labeled: green - Isl1 and Isl2; red - Tacr1 (assay MGI:6755482).
Specimen
3B:
(close )
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Genetic Background:
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involves: C57BL/6J * NMRI
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Isl1 and Isl2; red - Tacr1 (assay MGI:6755482).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
3B left
,
3B right
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Expression is in the nucleus ambiguous and is absent in the embryonic parafacial respiratory group.
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| TS24: facial nucleus |
Present |
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3B left
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3B right
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Specimen
3C:
embryonic day 16.5; ScribCrc/Scrib +
(more )
Note: Double labeled: green - Isl1 and Isl2; red - Tacr1 (assay MGI:6755482).
Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL/6J * NMRI
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Mutant Allele(s):
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ScribCrc/Scrib+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Isl1 and Isl2; red - Tacr1 (assay MGI:6755482).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
3C right
,
3C left
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Expression is in the nucleus ambiguous and is absent in the embryonic parafacial respiratory group.
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| TS24: facial nucleus |
Present |
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3C left
,
3C right
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Specimen
3D:
embryonic day 16.5; ScribCrc/ ScribCrc
(more )
Note: Double labeled: green - Isl1 and Isl2; red - Tacr1 (assay MGI:6755482).
Specimen
3D:
(close )
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Genetic Background:
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involves: C57BL/6J * NMRI
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Mutant Allele(s):
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ScribCrc/ScribCrc
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Isl1 and Isl2; red - Tacr1 (assay MGI:6755482).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
3D left
,
3D right
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Expression is in the nucleus ambiguous and is absent in the embryonic parafacial respiratory group.
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| TS24: facial nucleus |
Present |
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3D left
,
3D right
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Analysis Tools