Smad6 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:266234 Wylie LA, et al., Developmental SMAD6 loss leads to blood vessel hemorrhage and disrupted endothelial cell junctions. Dev Biol. 2018 Oct 15;442(2):199-209
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6753279
Gene symbol:	Smad6
Gene name:	SMAD family member 6

Reporter:	lacZ
	Direct Detection

Results

2A ©

Specimen 2A: embryonic day 9.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS15: heart	Present		2A
TS15: outflow tract	Present		2A

2B ©

Specimen 2B: embryonic day 10.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		2B
TS17: heart	Present		2B

2C ©

Specimen 2C: embryonic day 10.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present	Regionally restricted	2C	Expression in descending aorta

2D ©

Specimen 2D: embryonic day 13.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS21: dorsal aorta	Present	Regionally restricted	2D	Expression in descending aorta
TS21: artery	Present	Regionally restricted	2D	Expression in branchial arch arteries.

2E ©

Specimen 2E: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: descending aorta	Present		2E
TS23: intercostal artery	Present		2E

2G ©

Specimen 2G: embryonic day 17.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS25: descending aorta	Present		2G

2H ©

Specimen 2H: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: common carotid artery	Present		2H
TS23: subclavian artery	Present		2H

2I ©

Specimen 2I: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: head blood vessel	Present		2I

2J ©

Specimen 2J: postnatal day 2; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS27: basilar artery	Strong		2J
TS27: middle cerebral artery	Strong		2J

2K ©

Specimen 2K: postnatal day 3; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS27: middle cerebral artery	Strong		2K

2M ©

2M inset ©

Specimen 2M: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: descending aorta	Present	Regionally restricted	2M , 2M inset	Expression localized to inner layer of dorsal aorta.

2N ©

2N inset ©

Specimen 2N: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: vertebral artery	Present		2N , 2N inset

2O ©

2O inset ©

Specimen 2O: postnatal day 2; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Present		2O inset , 2O

2P ©

2P inset ©

Specimen 2P: postnatal day 3; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Present		2P inset , 2P

2Q ©

Specimen 2Q: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: tibia	Absent		2Q	No expression in cartilage of tibia.

2R ©

Specimen 2R: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: outflow tract	Present	Regionally restricted	2R

2S ©

Specimen 2S: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: lung	Absent		2S

2T ©

Specimen 2T: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: pancreas	Absent		2T
TS23: stomach	Absent		2T

2U ©

Specimen 2U: embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)

Structure	Level	Pattern	Image	Note
TS23: aorta	Present		2U
TS23: metanephros	Absent		2U

2V'' ©

2V''' ©

2V''' inset ©

2V'''' ©

Specimen 2V'',V''',V'''': embryonic day 15.5; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)
Note: Double labeled: light blue - Smad6 reporter; black - SMA (Acta2 assay MGI:6753287).

Structure	Level	Pattern	Image	Note
TS23: aorta	Present	Regionally restricted	2V'''' , 2V'' , 2V''' inset , 2V'''	Co-localization with Pecam1 in endothelial cells. No overlap with SMA (Acta2) or NG2 (Cspg4) expression.

2W'' ©

2W''' ©

2W''' inset ©

2W'''' ©

Specimen 2W'',W''',W'''': postnatal day 3; Smad6^tm1Glvn/Smad6^tm1Glvn (more

)
Note: Double labeled: light blue - Smad6 reporter; black - SMA (Acta2 assay MGI:6753287).

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Present	Regionally restricted	2W''' , 2W'''' , 2W'' , 2W''' inset	Co-localization with Pecam1 in endothelial cells. No overlap with SMA (Acta2) or NG2 (Cspg4) expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24